Photoactivated disinfection (PAD) of dental root canal system – An ex-vivo study

To investigate the efficacy of photo activated disinfection (PAD) in reducing colony-forming unit (CFU) counts of Enterococcus faecalis (E. faecalis) in infected dental root canals. The study compared the efficacy of PAD with conventional endodontic treatment (CET) and also a combination of CET alon...

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Bibliographic Details
Published in:Saudi journal of biological sciences 2016-01, Vol.23 (1), p.122-127
Main Authors: Mohan, Dennis, Maruthingal, Sunith, Indira, Rajamani, Divakar, Darshan Devang, Al Kheraif, Abdulaziz Abdullah, Ramakrishnaiah, Ravikumar, Durgesh, B.H., Basavarajappa, Santhosh, John, Jacob
Format: Article
Language:English
Subjects:
Chemo-mechanical preparation (CMP)
Colony forming unit (CFU)
Conventional endodontic treatment (CET)
Original
Photo activated disinfection (PAD)
Photosensitiser (PS)
Tryptone Soya Broth (TSB) agar medium
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Summary:To investigate the efficacy of photo activated disinfection (PAD) in reducing colony-forming unit (CFU) counts of Enterococcus faecalis (E. faecalis) in infected dental root canals. The study compared the efficacy of PAD with conventional endodontic treatment (CET) and also a combination of CET along with PAD. 53 maxillary incisors were taken for the study. Teeth were divided into 3 groups, CET (Group I) (n=11), PAD (Group II) (n=21), and a combination of CET and PAD (Group III) which consisted of (n=21) samples, Group II and Group III were further divided into 2 subgroups, Group IIa, IIb and Group IIIa, IIIb. Strains of E. faecalis were inoculated in all the root canals. CET group samples were treated by chemo-mechanical preparation (CMP) alone, PAD samples were treated with laser alone at 2 different exposure time (4min and 2min). In the combination treatment, samples were treated initially by CET and then by PAD for a time period of 4min and 2min. Contents of the root canal were aspirated, diluted and plated in Tryptone Soya Broth (TSB) and plates were incubated for 24h to observe the bacterial regrowth. Showed PAD used along with CMP reduced the bacterial load of E. faecalis by 99.5% at 4min and 98.89% at 2min. PAD may be an adjunctive procedure to kill residual bacteria in the dental root canal systems after standard endodontic root canal preparation.
ISSN:1319-562X
2213-7106
DOI:10.1016/j.sjbs.2015.01.013