Transcriptome profiling of bovine inner cell mass and trophectoderm derived from in vivo generated blastocysts

This study describes the generation and analysis of the transcriptional profile of bovine inner cell mass (ICM) and trophectoderm (TE), obtained from in vivo developed embryos by using a bovine-embryo specific array (EmbryoGENE) containing 37,238 probes. A total of 4,689 probes were differentially e...

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Bibliographic Details
Published in:BMC developmental biology 2015-12, Vol.15 (49), p.49-49, Article 49
Main Authors: Hosseini, S M, Dufort, I, Caballero, J, Moulavi, F, Ghanaei, H R, Sirard, M A
Format: Article
Language:English
Subjects:
Analysis
Animals
Blastocyst
Blastocyst Inner Cell Mass - cytology
Cattle
Cell Differentiation - genetics
Cell Lineage
Developmental biology
Ectoderm - cytology
Ectoderm - embryology
Embryo, Mammalian - embryology
Embryonic Development - genetics
Female
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Developmental - genetics
Influence
Methyltransferases
Microarray Analysis
Polymerase Chain Reaction
Transcription, Genetic - genetics
Transcriptional Activation - genetics
Transcriptome - genetics
Trophoblasts - cytology
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Summary:This study describes the generation and analysis of the transcriptional profile of bovine inner cell mass (ICM) and trophectoderm (TE), obtained from in vivo developed embryos by using a bovine-embryo specific array (EmbryoGENE) containing 37,238 probes. A total of 4,689 probes were differentially expressed between ICM and TE, among these, 2,380 and 2,309 probes were upregulated in ICM and TE tissues, respectively (P ≤ 0.01, FC ≥ 2.0, FDR: 2.0). Ontological classification of the genes predominantly expressed in ICM emerged a range of functional categories with a preponderance of genes involved in basal and developmental signaling pathways including P53, TGFβ, IL8, mTOR, integrin, ILK, and ELF2 signalings. Cross-referencing of microarray data with two available in vitro studies indicated a marked reduction in ICM vs. TE transcriptional difference following in vitro culture of bovine embryos. Moreover, a great majority of genes that were found to be misregulated following in vitro culture of bovine embryos were known genes involved in epigenetic regulation of pluripotency and cell differentiation including DNMT1, GADD45, CARM1, ELF5 HDAC8, CCNB1, KDM6A, PRDM9, CDX2, ARID3A, IL6, GADD45A, FGFR2, PPP2R2B, and SMARCA2. Cross-species referencing of microarray data revealed substantial divergence between bovine and mouse and human in signaling pathways involved in early lineage specification. The transcriptional changes occur during ICM and TE lineages specification in bovine is greater than previously understood. Therefore, this array data establishes a standard to evaluate the in vitro imprint on the transcriptome and to hypothesize the cross-species differences that allow in vitro acquisition of pluripotent ICM in human and mice but hinder that process in bovine.
ISSN:1471-213X
1471-213X
DOI:10.1186/s12861-015-0096-3