Study of model systems to test the potential function of Artemia group 1 late embryogenesis abundant (LEA) proteins

Embryos of the brine shrimp, Artemia franciscana, are genetically programmed to develop either ovoviparously or oviparously depending on environmental conditions. Shortly upon their release from the female, ovipartfus embryos enter diapause during which time they undergo major metabolic rate depress...

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Bibliographic Details
Published in:Cell stress & chaperones 2016-01, Vol.21 (1), p.139-154
Main Authors: Warner, Alden H., Guo, Zhi-hao, Moshi, Sandra, Hudson, John W., Kozarova, Anna
Format: Article
Language:English
Subjects:
Adaptation, Physiological - genetics
Adaptation, Physiological - physiology
Animals
Artemia
Artemia - embryology
Artemia - genetics
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cancer Research
Cell Biology
Cell growth
Desiccation
Diapause
Embryo, Nonmammalian - metabolism
Embryogenesis
Embryonic Development
Embryos
Escherichia coli - genetics
Escherichia coli - metabolism
Freezing
Gels
Gene Expression Regulation
Genetic vectors
Immunology
Models, Biological
Neurosciences
Open reading frames
Original Paper
Osmotic Pressure - physiology
Plasmids
Proteome - analysis
Proteome - genetics
Stress, Physiological - genetics
Stress, Physiological - physiology
Yeasts
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Summary:Embryos of the brine shrimp, Artemia franciscana, are genetically programmed to develop either ovoviparously or oviparously depending on environmental conditions. Shortly upon their release from the female, ovipartfus embryos enter diapause during which time they undergo major metabolic rate depression while simultaneously synthesize proteins that permit them to tolerate a wide range of stressful environmental events including prolonged periods of desiccation, freezing, and anoxia. Among the known stress-related proteins that accumulate in embryos entering diapause are the late embryogenesis abundant (LEA) proteins. This large group of intrinsically disordered proteins has been proposed to act as molecular shields or chaperones of macromolecules which are otherwise intolerant to harsh conditions associated with diapause. In this research, we used two model systems to study the potential function of the group 1 LEA proteins from Artemia. Expression of the Artemia group 1 gene (AfrLEA-1) in Escherichia coli inhibited growth in proportion to the number of 20-mer amino acid motifs expressed. As well, clones of E. coli, transformed with the AfrLEA-1 gene, expressed multiple bands of LEA proteins, either intrinsically or upon induction with isopropyl-β-thiogalactoside (IPTG), in a vector-specific manner. Expression of AfrLEA-1 in E. coli did not overcome the inhibitory effects of high concentrations of NaC1 and KC1 but modulated growth inhibition resulting from high concentrations of sorbitol in the growth medium. In contrast, expression of the AfrLEA-1 gene in Saccharomyces cerevisiae did not alter the growth kinetics or permit yeast to tolerate high concentrations of NaC1, KC1, or sorbitol. However, expression of AfrLEA-1 in yeast improved its tolerance to drying (desiccation) and freezing. Under our experimental conditions, both E. coli and S. cerevisiae appear to be potentially suitable hosts to study the function of Artemia group 1 LEA proteins under environmentally stressful conditions.
ISSN:1355-8145
1466-1268
DOI:10.1007/s12192-015-0647-3