Identification of Putative Carboxylesterase and Glutathione S-transferase Genes from the Antennae of the Chilo suppressalis (Lepidoptera: Pyralidae)

In insects, rapid degradation of odorants in antennae is extremely important for the sensitivity of olfactory receptor neurons. Odorant degradation in insect antennae is mediated by multiple enzymes, especially the carboxylesterases (CXEs) and glutathione S-transferases (GSTs). The Asiatic rice bore...

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Bibliographic Details
Published in:Journal of insect science (Tucson, Ariz.) Ariz.), 2015, Vol.15 (1), p.103
Main Authors: Liu, Su, Gong, Zhong-Jun, Rao, Xiang-Jun, Li, Mao-Ye, Li, Shi-Guang
Format: Article
Language:English
Subjects:
Animals
Arthropod Antennae - metabolism
Carboxylesterase - genetics
Carboxylesterase - metabolism
Female
Glutathione Transferase - genetics
Glutathione Transferase - metabolism
Insect Proteins - genetics
Insect Proteins - metabolism
Male
Molecular Sequence Data
Moths - genetics
Moths - metabolism
Phylogeny
Receptors, Odorant - genetics
Receptors, Odorant - metabolism
Sequence Analysis, DNA
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Summary:In insects, rapid degradation of odorants in antennae is extremely important for the sensitivity of olfactory receptor neurons. Odorant degradation in insect antennae is mediated by multiple enzymes, especially the carboxylesterases (CXEs) and glutathione S-transferases (GSTs). The Asiatic rice borer, Chilo suppressalis, is an economically important lepidopteran pest which causes great economic damage to cultivated rice crops in many Asian countries. In this study, we identified 19 putative CXE and 16 GST genes by analyzing previously constructed antennal transcriptomes of C. suppressalis. BLASTX best hit results showed that these genes are most homologous to their respective orthologs in other lepidopteran species. Phylogenetic analyses revealed that these CXE and GST genes were clustered into various clades. Reverse-transcription quantitative polymerase chain reaction assays showed that three CXE genes (CsupCXE8, CsupCXE13, and CsupCXE18) are antennae-enriched. These genes are candidates for involvement in odorant degradation. Unexpectedly, none of the GST genes were found to be antennae-specific. Our results pave the way for future researches of the odorant degradation mechanism of C. suppressalis at the molecular level.
ISSN:1536-2442
1536-2442
DOI:10.1093/jisesa/iev082