Exercise training restores the cardiac microRNA-1 and -214 levels regulating Ca2+ handling after myocardial infarction

Impaired cardiomyocyte contractility and calcium handling are hallmarks of left ventricular contractile dysfunction. Exercise training has been used as a remarkable strategy in the treatment of heart disease. The microRNA-1, which targets sodium/calcium exchanger 1 (NCX), and microRNA-214, which tar...

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Bibliographic Details
Published in:BMC cardiovascular disorders 2015-12, Vol.15 (1), p.166-166, Article 166
Main Authors: Melo, Stéphano Freitas Soares, Barauna, Valério Garrone, Neves, Vander José, Fernandes, Tiago, Lara, Lucienne da Silva, Mazzotti, Diego Robles, Oliveira, Edilamar Menezes
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
Animals
Calcium - metabolism
Calcium Signaling
Disease Models, Animal
Exercise Therapy
Gene expression
Gene Expression Regulation
Heart
Heart attack
Male
MicroRNA
MicroRNAs - genetics
MicroRNAs - metabolism
Myocardial Infarction - genetics
Myocardial Infarction - metabolism
Myocardial Infarction - physiopathology
Myocardial Infarction - therapy
Myocardium - metabolism
Physical fitness
Rats, Wistar
Recovery of Function
Sarcoplasmic Reticulum Calcium-Transporting ATPases - genetics
Sarcoplasmic Reticulum Calcium-Transporting ATPases - metabolism
Sodium-Calcium Exchanger - genetics
Sodium-Calcium Exchanger - metabolism
Time Factors
Ventricular Function, Left
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Summary:Impaired cardiomyocyte contractility and calcium handling are hallmarks of left ventricular contractile dysfunction. Exercise training has been used as a remarkable strategy in the treatment of heart disease. The microRNA-1, which targets sodium/calcium exchanger 1 (NCX), and microRNA-214, which targets sarcoplasmic reticulum calcium ATPase-2a (Serca2a), are involved in cardiac function regulation. Thus, the aim of this study was to evaluate the effect of exercise training on cardiac microRNA-1 and -214 expression after myocardial infarction. Wistar rats were randomized into four groups: sedentary sham (S-SHAM), sedentary infarction (S-INF), trained sham (T-SHAM), and trained infarction (T-INF). Exercise training consisted of 60 min/days, 5 days/week for 10 weeks with 3 % of body weight as overload beginning four weeks after myocardial infarction. MicroRNA-1 and -214 expressions were, respectively, decreased (52 %) and increased (54 %) in the S-INF compared to the S-SHAM, while exercise training normalized the expression of these microRNAs. The microRNA targets NCX and Serca-2a protein expression were, respectively, decreased (55 %) and increased (34 %) in the T-INF group compared to the S-INF group. These results suggest that exercise training restores microRNA-1 and -214 expression levels and prevents change in both NCX and Serca-2a protein and gene expressions. Altogether, our data suggest a molecular mechanism to restore ventricular function after exercise training in myocardial infarction rats.
ISSN:1471-2261
1471-2261
DOI:10.1186/s12872-015-0156-4