Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI

CD163 is a membrane receptor expressed by macrophage lineage. Studies performed in atherosclerosis have shown that CD163 expression is increased at inflammatory sites, pointing at the presence of intraplaque hemorrhagic sites or asymptomatic plaques. Hence, imaging of CD163 expressing macrophages is...

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Bibliographic Details
Published in:Scientific reports 2015-11, Vol.5 (1), p.17135-17135, Article 17135
Main Authors: Tarin, Carlos, Carril, Monica, Martin-Ventura, Jose Luis, Markuerkiaga, Irati, Padro, Daniel, Llamas-Granda, Patricia, Moreno, Juan Antonio, García, Isabel, Genicio, Nuria, Plaza-Garcia, Sandra, Blanco-Colio, Luis Miguel, Penades, Soledad, Egido, Jesus
Format: Article
Language:English
Subjects:
Animals
Antigens, CD - metabolism
Antigens, Differentiation, Myelomonocytic - metabolism
Atherosclerosis - diagnosis
Atherosclerosis - metabolism
Atherosclerosis - pathology
Cell Line
Disease Models, Animal
Ferrous Compounds - chemistry
Gold - chemistry
Macrophages - metabolism
Magnetic Resonance Imaging - methods
Magnetite Nanoparticles - chemistry
Magnetite Nanoparticles - toxicity
Magnetite Nanoparticles - ultrastructure
Mice
Mice, Knockout
Plaque, Atherosclerotic - diagnosis
Plaque, Atherosclerotic - metabolism
Plaque, Atherosclerotic - pathology
Receptors, Cell Surface - metabolism
Reproducibility of Results
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Summary:CD163 is a membrane receptor expressed by macrophage lineage. Studies performed in atherosclerosis have shown that CD163 expression is increased at inflammatory sites, pointing at the presence of intraplaque hemorrhagic sites or asymptomatic plaques. Hence, imaging of CD163 expressing macrophages is an interesting strategy in order to detect atherosclerotic plaques. We have prepared a targeted probe based on gold-coated iron oxide nanoparticles vectorized with an anti-CD163 antibody for the specific detection of CD163 by MRI. Firstly, the specificity of the targeted probe was validated in vitro by incubation of the probe with CD163(+) or (-) macrophages. The probe was able to selectively detect CD163(+) macrophages both in human and murine cells. Subsequently, the targeted probe was injected in 16 weeks old apoE deficient mice developing atherosclerotic lesions and the pararenal abdominal aorta was imaged by MRI. The accumulation of probe in the site of interest increased over time and the signal intensity decreased significantly 48 hours after the injection. Hence, we have developed a highly sensitive targeted probe capable of detecting CD163-expressing macrophages that could provide useful information about the state of the atheromatous lesions.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep17135