Development of a High-Throughput and Miniaturized Cytokinesis-Block Micronucleus Assay for Use as a Biological Dosimetry Population Triage Tool

Biodosimetry is an essential tool for providing timely assessments of radiation exposure. For a large mass-casualty event involving exposure to ionizing radiation, it is of utmost importance to rapidly provide dose information for medical treatment. The well-established cytokinesis-block micronucleu...

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Bibliographic Details
Published in:Radiation research 2015-08, Vol.184 (2), p.134-142
Main Authors: Lue, Stanley W., Repin, Mikhail, Mahnke, Ryan, Brenner, David J.
Format: Article
Language:English
Subjects:
Assaying
Assessments
Biological
Cytokinesis - radiation effects
Dosimetry
High-Throughput Screening Assays
Humans
Ionizing radiation
Medical services
Micronucleus Tests
Radiation effects
Radiation, Ionizing
Radiometry - instrumentation
Radiometry - methods
REGULAR ARTICLES
Space life sciences
Triage
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Summary:Biodosimetry is an essential tool for providing timely assessments of radiation exposure. For a large mass-casualty event involving exposure to ionizing radiation, it is of utmost importance to rapidly provide dose information for medical treatment. The well-established cytokinesis-block micronucleus (CBMN) assay is a validated method for biodosimetry. However, the need for an accelerated sample processing is required for the CBMN assay to be a suitable population triage tool. We report here on the development of a high-throughput and miniaturized version of the CMBN assay for accelerated sample processing.
ISSN:0033-7587
1938-5404
DOI:10.1667/RR13991.1