Multimodal Imaging Reveals Temporal and Spatial Microglia and Matrix Metalloproteinase Activity after Experimental Stroke

Stroke is the most common cause of death and disability from neurologic disease in humans. Activation of microglia and matrix metalloproteinases (MMPs) is involved in positively and negatively affecting stroke outcome. Novel, noninvasive, multimodal imaging methods visualizing microglial and MMP alt...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cerebral blood flow and metabolism 2015-11, Vol.35 (11), p.1711-1721
Main Authors: Zinnhardt, Bastian, Viel, Thomas, Wachsmuth, Lydia, Vrachimis, Alexis, Wagner, Stefan, Breyholz, Hans-Jörg, Faust, Andreas, Hermann, Sven, Kopka, Klaus, Faber, Cornelius, Dollé, Frédéric, Pappata, Sabina, Planas, Anna M, Tavitian, Bertrand, Schäfers, Michael, Sorokin, Lydia M, Kuhlmann, Michael T, Jacobs, Andreas H
Format: Article
Language:English
Subjects:
Animals
Immunohistochemistry
Infarction, Middle Cerebral Artery - diagnostic imaging
Infarction, Middle Cerebral Artery - metabolism
Magnetic Resonance Imaging
Matrix Metalloproteinases - metabolism
Mice
Mice, Inbred C57BL
Microglia - diagnostic imaging
Microglia - pathology
Multimodal Imaging - methods
Neuroimaging - methods
Original
Positron-Emission Tomography
Pyrazoles
Pyrimidines
Radiopharmaceuticals
Stroke - diagnostic imaging
Stroke - enzymology
Stroke - pathology
Technetium Tc 99m Exametazime
Tomography, Emission-Computed, Single-Photon
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Stroke is the most common cause of death and disability from neurologic disease in humans. Activation of microglia and matrix metalloproteinases (MMPs) is involved in positively and negatively affecting stroke outcome. Novel, noninvasive, multimodal imaging methods visualizing microglial and MMP alterations were employed. The spatio-temporal dynamics of these parameters were studied in relation to blood flow changes. Micro positron emission tomography (μPET) using [18F]BR-351 showed MMP activity within the first days after transient middle cerebral artery occlusion (tMCAo), followed by increased [18F]DPA-714 uptake as a marker for microglia activation with a maximum at 14 days after tMCAo. The inflammatory response was spatially located in the infarct core and in adjacent (penumbral) tissue. For the first time, multimodal imaging based on PET, single photon emission computed tomography, and magnetic resonance imaging revealed insight into the spatio-temporal distribution of critical parameters of poststroke inflammation. This allows further evaluation of novel treatment paradigms targeting the postischemic inflammation.
ISSN:0271-678X
1559-7016
DOI:10.1038/jcbfm.2015.149