A cytoplasmic pathway for gapmer antisense oligonucleotide-mediated gene silencing in mammalian cells

Antisense oligonucleotides (ASOs) are known to trigger mRNA degradation in the nucleus via an RNase H-dependent mechanism. We have now identified a putative cytoplasmic mechanism through which ASO gapmers silence their targets when transfected or delivered gymnotically (i.e. in the absence of any tr...

Full description

Bibliographic Details

Published in:	Nucleic acids research 2015-10, Vol.43 (19), p.9350-9361
Main Authors:	Castanotto, Daniela, Lin, Min, Kowolik, Claudia, Wang, LiAnn, Ren, Xiao-Qin, Soifer, Harris S, Koch, Troels, Hansen, Bo Rode, Oerum, Henrik, Armstrong, Brian, Wang, Zhigang, Bauer, Paul, Rossi, John, Stein, C A
Format:	Article
Language:	English
Subjects:	Argonaute Proteins - metabolism Cell Line Cytoplasm - chemistry Cytoplasm - metabolism Gene Silencing Gene Transfer Techniques Molecular Biology Oligonucleotides, Antisense - analysis Oligonucleotides, Antisense - chemistry Oligonucleotides, Antisense - metabolism RNA, Messenger - metabolism RNA, Small Interfering Transfection
Citations:	Items that this one cites Items that cite this one
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!