Structure of RC1339/APRc from Rickettsia conorii, a retropepsin-like aspartic protease

The crystal structures of two constructs of RC1339/APRc from Rickettsia conorii, consisting of either residues 105–231 or 110–231 followed by a His tag, have been determined in three different crystal forms. As predicted, the fold of a monomer of APRc resembles one‐half of the mandatory homodimer of...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2015-10, Vol.71 (10), p.2109-2118
Main Authors: Li, Mi, Gustchina, Alla, Cruz, Rui, Simões, Marisa, Curto, Pedro, Martinez, Juan, Faro, Carlos, Simões, Isaura, Wlodawer, Alexander
Format: Article
Language:English
Subjects:
Aspartic Acid Proteases - chemistry
aspartic proteases
Bacteria
bacterial proteases
Bacterial Proteins - chemistry
Crystal structure
Crystallization
Crystallography, X-Ray
dimeric proteins
Dimers
enzyme evolution
Molecular structure
Monomers
Pepsin A - chemistry
Protease
Protein Conformation
Protein Multimerization
Research Papers
Residues
Rickettsia conorii - chemistry
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Summary:The crystal structures of two constructs of RC1339/APRc from Rickettsia conorii, consisting of either residues 105–231 or 110–231 followed by a His tag, have been determined in three different crystal forms. As predicted, the fold of a monomer of APRc resembles one‐half of the mandatory homodimer of retroviral pepsin‐like aspartic proteases (retropepsins), but the quaternary structure of the dimer of APRc differs from that of the canonical retropepsins. The observed dimer is most likely an artifact of the expression and/or crystallization conditions since it cannot support the previously reported enzymatic activity of this bacterial aspartic protease. However, the fold of the core of each monomer is very closely related to the fold of retropepsins from a variety of retroviruses and to a single domain of pepsin‐like eukaryotic enzymes, and may represent a putative common ancestor of monomeric and dimeric aspartic proteases.
ISSN:1399-0047
0907-4449
2059-7983
1399-0047
2059-7983
DOI:10.1107/S1399004715013905