Direct profiling of the phospholipid composition of adult Caenorhabditis elegans using whole-body imaging mass spectrometry

A protocol for the direct analysis of the phospholipid composition in the whole body of adult soil nematode, Caenorhabditis elegans (C. elegans), was developed, which combined freeze-cracking of the exoskeletal cuticle and matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2015, Vol.407 (25), p.7589-7602
Main Authors: Hameed, Saira, Ikegami, Koji, Sugiyama, Eiji, Matsushita, Shoko, Kimura, Yoshishige, Hayasaka, Takahiro, Sugiura, Yuki, Masaki, Noritaka, Waki, Michihiko, Ohta, Isao, Hossen, Md Amir, Setou, Mitsutoshi
Format: Article
Language:English
Subjects:
Adults
Analytical Chemistry
Animals
Biochemistry
Biomolecules
Caenorhabditis elegans
Caenorhabditis elegans - chemistry
Caenorhabditis elegans - genetics
Caenorhabditis elegans - ultrastructure
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Encoding
fatty acid composition
Fatty acids
Fatty Acids - analysis
Fatty Acids - genetics
Food Science
Freezing
genes
Genetics
Identification and classification
image analysis
Laboratory Medicine
Lasers
Mass spectrometry
Methods
Monitoring/Environmental Analysis
mutants
Nematodes
New Applications of Mass Spectrometry in Biomedicine
omega-3 fatty acids
Peptides
phosphatidylcholines
Phospholipids
Phospholipids - analysis
Phospholipids - genetics
Physiological aspects
Profiling
Research Paper
Scientific imaging
soil nematodes
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
stearoyl-CoA desaturase
sublimation
Whole Body Imaging - methods
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Summary:A protocol for the direct analysis of the phospholipid composition in the whole body of adult soil nematode, Caenorhabditis elegans (C. elegans), was developed, which combined freeze-cracking of the exoskeletal cuticle and matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS). Biomolecules in the m/z range from 700 to 900 were more effectively detected in the freeze-cracked than from simple frozen adult nematode bodies. Different distribution of biomolecules was observed in a nematode body when the matrix was applied with a sublimation deposition method. The whole-body IMS technique was applied on genetically deficient mutant C. elegans to combine whole-body lipidomics and genetics, by comparing the fatty acid compositions, especially of the phosphatidylcholine (PC) species, between the wild-type and fat-1 mutants, which lack the gene encoding an n-3 fatty acid desaturase. A significant reduction of PC(20:5/20:5) and PC(20:4/20:5) and a marked increase of PC(20:4/20:4), PC(20:3/20:4), and PC(20:3/20:3) were detected in the fat-1 mutants in positive ion mode. In addition, phospholipid compositions other than PCs were analyzed in negative ion mode. A loss of a possible phosphatidylinositol (PI) with 18:0/20:5 and a compensative accumulation of putative PI(18:0/20:4) were detected in the fat-1 mutants. In conclusion, the whole-body MALDI-IMS technique is useful for the profiling of multiple biomolecules in C. elegans in both intra- and inter-individual levels.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-015-8932-7