Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery

Precise fluorescence-guided surgery (FGS) for pancreatic cancer has the potential to greatly improve the outcome in this recalcitrant disease. To achieve this goal, we have used genetic reporters to color code cancer and stroma cells in a patient-derived orthotopic xenograft (PDOX) model. The telome...

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Bibliographic Details
Published in:Cancer gene therapy 2015-07, Vol.22 (7), p.344-350
Main Authors: Yano, S, Hiroshima, Y, Maawy, A, Kishimoto, H, Suetsugu, A, Miwa, S, Toneri, M, Yamamoto, M, Katz, M H G, Fleming, J B, Urata, Y, Tazawa, H, Kagawa, S, Bouvet, M, Fujiwara, T, Hoffman, R M
Format: Article
Language:English
Subjects:
Adenovirus
Animals
Care and treatment
Color
Diagnosis
Gene therapy
Genes, Reporter
Genetic aspects
Green fluorescent protein
Green Fluorescent Proteins - biosynthesis
Green Fluorescent Proteins - genetics
Health aspects
Luminescent Proteins - biosynthesis
Luminescent Proteins - genetics
Mice, Nude
Mice, Transgenic
Neoplasm Recurrence, Local - prevention & control
Neoplasm Transplantation
Pancreatic cancer
Pancreatic Neoplasms - metabolism
Pancreatic Neoplasms - pathology
Pancreatic Neoplasms - surgery
Physiological aspects
Red Fluorescent Protein
Stromal cells
Surgery
Surgery, Computer-Assisted
Telomerase
Transgenic mice
Tumors
Xenografts
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Summary:Precise fluorescence-guided surgery (FGS) for pancreatic cancer has the potential to greatly improve the outcome in this recalcitrant disease. To achieve this goal, we have used genetic reporters to color code cancer and stroma cells in a patient-derived orthotopic xenograft (PDOX) model. The telomerase-dependent green fluorescent protein (GFP)-containing adenovirus OBP-401 was used to label the cancer cells of a pancreatic cancer PDOX. The PDOX was previously grown in a red fluorescent protein (RFP) transgenic mouse that stably labeled the PDOX stroma cells bright red. The color-coded PDOX model enabled FGS to completely resect the pancreatic tumors including stroma. Dual-colored FGS significantly prevented local recurrence, which bright-light surgery or single-color FGS could not. FGS, with color-coded cancer and stroma cells has important potential for improving the outcome of recalcitrant-cancer surgery.
ISSN:0929-1903
1476-5500
DOI:10.1038/cgt.2015.26