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hTAF(II)68, a novel RNA/ssDNA‐binding protein with homology to the pro‐oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II
TFIID is the main sequence‐specific DNA‐binding component of the RNA polymerase II (Pol II) transcriptional machinery. It is a multiprotein complex composed of the TATA‐binding protein (TBP) and TBP‐associated factors (TAF(II)s). Here we report the cloning and characterization of a novel human TBP‐a...
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| Published in: | The EMBO journal 1996-09, Vol.15 (18), p.5022-5031 |
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| Main Authors: | , , , , |
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| Language: | English |
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| container_end_page | 5031 |
| container_issue | 18 |
| container_start_page | 5022 |
| container_title | The EMBO journal |
| container_volume | 15 |
| creator | Bertolotti, A. Lutz, Y. Heard, D. J. Chambon, P. Tora, L. |
| description | TFIID is the main sequence‐specific DNA‐binding component of the RNA polymerase II (Pol II) transcriptional machinery. It is a multiprotein complex composed of the TATA‐binding protein (TBP) and TBP‐associated factors (TAF(II)s). Here we report the cloning and characterization of a novel human TBP‐associated factor, hTAF(II)68. It contains a consensus RNA‐binding domain (RNP‐CS) and binds not only RNA, but also single stranded (ss) DNA. hTAF(II)68 shares extensive sequence similarity with TLS/FUS and EWS, two human nuclear RNA‐binding pro‐oncoproteins which are products of genes commonly translocated in human sarcomas. Like hTAF(II)68, TLS/FUS is also associated with a sub‐population of TFIID complexes chromatographically separable from those containing hTAF(II)68. Therefore, these RNA and/or ssDNA‐binding proteins may play specific roles during transcription initiation at distinct promoters. Moreover, we demonstrate that hTAF(II)68 co‐purifies also with the human RNA polymerase II and can enter the preinitiation complex together with Pol II. |
| doi_str_mv | 10.1002/j.1460-2075.1996.tb00882.x |
| format | article |
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Therefore, these RNA and/or ssDNA‐binding proteins may play specific roles during transcription initiation at distinct promoters. Moreover, we demonstrate that hTAF(II)68 co‐purifies also with the human RNA polymerase II and can enter the preinitiation complex together with Pol II.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1002/j.1460-2075.1996.tb00882.x</identifier><identifier>PMID: 8890175</identifier><language>eng</language><publisher>England: EMBO Press</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biochemistry, Molecular Biology ; DNA Polymerase II - metabolism ; DNA, Single-Stranded - metabolism ; Electrophoresis, Polyacrylamide Gel ; HeLa Cells ; Heterogeneous-Nuclear Ribonucleoproteins ; Humans ; Life Sciences ; Molecular Sequence Data ; Neoplasm Proteins - chemistry ; Neoplasm Proteins - metabolism ; Ribonucleoproteins - chemistry ; Ribonucleoproteins - metabolism ; RNA - metabolism ; RNA, Heterogeneous Nuclear - metabolism ; RNA-Binding Protein EWS ; RNA-Binding Protein FUS ; RNA-Binding Proteins - chemistry ; RNA-Binding Proteins - metabolism ; Sequence Alignment ; TATA-Binding Protein Associated Factors ; Transcription Factor TFIID ; Transcription Factors - chemistry ; Transcription Factors - genetics ; Transcription Factors - metabolism</subject><ispartof>The EMBO journal, 1996-09, Vol.15 (18), p.5022-5031</ispartof><rights>1996 European Molecular Biology Organization</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5122-9828187877185efc2662c042a9bc523f6d24d5e3853bdda990887eb1c7ea97c03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC452240/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC452240/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,27957,27958,53827,53829</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8890175$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03829843$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Bertolotti, A.</creatorcontrib><creatorcontrib>Lutz, Y.</creatorcontrib><creatorcontrib>Heard, D. J.</creatorcontrib><creatorcontrib>Chambon, P.</creatorcontrib><creatorcontrib>Tora, L.</creatorcontrib><title>hTAF(II)68, a novel RNA/ssDNA‐binding protein with homology to the pro‐oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><description>TFIID is the main sequence‐specific DNA‐binding component of the RNA polymerase II (Pol II) transcriptional machinery. It is a multiprotein complex composed of the TATA‐binding protein (TBP) and TBP‐associated factors (TAF(II)s). Here we report the cloning and characterization of a novel human TBP‐associated factor, hTAF(II)68. It contains a consensus RNA‐binding domain (RNP‐CS) and binds not only RNA, but also single stranded (ss) DNA. hTAF(II)68 shares extensive sequence similarity with TLS/FUS and EWS, two human nuclear RNA‐binding pro‐oncoproteins which are products of genes commonly translocated in human sarcomas. Like hTAF(II)68, TLS/FUS is also associated with a sub‐population of TFIID complexes chromatographically separable from those containing hTAF(II)68. Therefore, these RNA and/or ssDNA‐binding proteins may play specific roles during transcription initiation at distinct promoters. Moreover, we demonstrate that hTAF(II)68 co‐purifies also with the human RNA polymerase II and can enter the preinitiation complex together with Pol II.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biochemistry, Molecular Biology</subject><subject>DNA Polymerase II - metabolism</subject><subject>DNA, Single-Stranded - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>HeLa Cells</subject><subject>Heterogeneous-Nuclear Ribonucleoproteins</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Molecular Sequence Data</subject><subject>Neoplasm Proteins - chemistry</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Ribonucleoproteins - chemistry</subject><subject>Ribonucleoproteins - metabolism</subject><subject>RNA - metabolism</subject><subject>RNA, Heterogeneous Nuclear - metabolism</subject><subject>RNA-Binding Protein EWS</subject><subject>RNA-Binding Protein FUS</subject><subject>RNA-Binding Proteins - chemistry</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>Sequence Alignment</subject><subject>TATA-Binding Protein Associated Factors</subject><subject>Transcription Factor TFIID</subject><subject>Transcription Factors - chemistry</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqVUs9u0zActhBodINHQLI4ICYtqe38sT2JQ9haFlSGRDtxtBzHbVwlcYnTbr3xCNx5O56EZKkq4MbFtvz9-cn-PgBeY-RjhMh47eMwRh5BNPIx57HfZggxRvyHJ2B0hJ6CESIx9kLM-HNw6twaIRQxik_ACWMcYRqNwM9ikUzfpul5zC6ghLXd6RJ-uU3Gzl3fJr--_8hMnZt6BTeNbbWp4b1pC1jYypZ2tYethW2he7Cj2lrZA83BxWw-nt7NoaxzOPk6h8ZB6ZxVRrY6H1wy2y2LaZpeP7K6qXBjy32lG-k0TNMX4NlSlk6_POxn4G46WVzdeLPPH9KrZOapCBPicUYYZpRRilmkl4rEMVEoJJJnKiLBMs5JmEc6YFGQ5bnkvPsqqjOsqJacKhScgXeD72abVTpXum4bWYpNYyrZ7IWVRvyN1KYQK7sTYURI2OvPB33xj-ommYn-DgWMcBYGO9xx3xxmNfbbVrtWVMYpXZay1nbrBGUhiwkPO-LlQFSNda7Ry6MzRqJvgViLPmrRRy36FohDC8RDJ37154uO0kPsHZ4M-L0p9f4_nMXk0_uPj-fgN87Bw6o</recordid><startdate>19960916</startdate><enddate>19960916</enddate><creator>Bertolotti, A.</creator><creator>Lutz, Y.</creator><creator>Heard, D. J.</creator><creator>Chambon, P.</creator><creator>Tora, L.</creator><general>EMBO Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope></search><sort><creationdate>19960916</creationdate><title>hTAF(II)68, a novel RNA/ssDNA‐binding protein with homology to the pro‐oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II</title><author>Bertolotti, A. ; Lutz, Y. ; Heard, D. J. ; Chambon, P. ; Tora, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5122-9828187877185efc2662c042a9bc523f6d24d5e3853bdda990887eb1c7ea97c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biochemistry, Molecular Biology</topic><topic>DNA Polymerase II - metabolism</topic><topic>DNA, Single-Stranded - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>HeLa Cells</topic><topic>Heterogeneous-Nuclear Ribonucleoproteins</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Molecular Sequence Data</topic><topic>Neoplasm Proteins - chemistry</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Ribonucleoproteins - chemistry</topic><topic>Ribonucleoproteins - metabolism</topic><topic>RNA - metabolism</topic><topic>RNA, Heterogeneous Nuclear - metabolism</topic><topic>RNA-Binding Protein EWS</topic><topic>RNA-Binding Protein FUS</topic><topic>RNA-Binding Proteins - chemistry</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>Sequence Alignment</topic><topic>TATA-Binding Protein Associated Factors</topic><topic>Transcription Factor TFIID</topic><topic>Transcription Factors - chemistry</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bertolotti, A.</creatorcontrib><creatorcontrib>Lutz, Y.</creatorcontrib><creatorcontrib>Heard, D. J.</creatorcontrib><creatorcontrib>Chambon, P.</creatorcontrib><creatorcontrib>Tora, L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bertolotti, A.</au><au>Lutz, Y.</au><au>Heard, D. J.</au><au>Chambon, P.</au><au>Tora, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>hTAF(II)68, a novel RNA/ssDNA‐binding protein with homology to the pro‐oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II</atitle><jtitle>The EMBO journal</jtitle><addtitle>EMBO J</addtitle><date>1996-09-16</date><risdate>1996</risdate><volume>15</volume><issue>18</issue><spage>5022</spage><epage>5031</epage><pages>5022-5031</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>TFIID is the main sequence‐specific DNA‐binding component of the RNA polymerase II (Pol II) transcriptional machinery. It is a multiprotein complex composed of the TATA‐binding protein (TBP) and TBP‐associated factors (TAF(II)s). Here we report the cloning and characterization of a novel human TBP‐associated factor, hTAF(II)68. It contains a consensus RNA‐binding domain (RNP‐CS) and binds not only RNA, but also single stranded (ss) DNA. hTAF(II)68 shares extensive sequence similarity with TLS/FUS and EWS, two human nuclear RNA‐binding pro‐oncoproteins which are products of genes commonly translocated in human sarcomas. Like hTAF(II)68, TLS/FUS is also associated with a sub‐population of TFIID complexes chromatographically separable from those containing hTAF(II)68. Therefore, these RNA and/or ssDNA‐binding proteins may play specific roles during transcription initiation at distinct promoters. Moreover, we demonstrate that hTAF(II)68 co‐purifies also with the human RNA polymerase II and can enter the preinitiation complex together with Pol II.</abstract><cop>England</cop><pub>EMBO Press</pub><pmid>8890175</pmid><doi>10.1002/j.1460-2075.1996.tb00882.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0261-4189 |
| ispartof | The EMBO journal, 1996-09, Vol.15 (18), p.5022-5031 |
| issn | 0261-4189 1460-2075 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_452240 |
| source | PubMed (Medline) |
| subjects | Amino Acid Sequence Base Sequence Biochemistry, Molecular Biology DNA Polymerase II - metabolism DNA, Single-Stranded - metabolism Electrophoresis, Polyacrylamide Gel HeLa Cells Heterogeneous-Nuclear Ribonucleoproteins Humans Life Sciences Molecular Sequence Data Neoplasm Proteins - chemistry Neoplasm Proteins - metabolism Ribonucleoproteins - chemistry Ribonucleoproteins - metabolism RNA - metabolism RNA, Heterogeneous Nuclear - metabolism RNA-Binding Protein EWS RNA-Binding Protein FUS RNA-Binding Proteins - chemistry RNA-Binding Proteins - metabolism Sequence Alignment TATA-Binding Protein Associated Factors Transcription Factor TFIID Transcription Factors - chemistry Transcription Factors - genetics Transcription Factors - metabolism |
| title | hTAF(II)68, a novel RNA/ssDNA‐binding protein with homology to the pro‐oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II |
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