hTAF(II)68, a novel RNA/ssDNA‐binding protein with homology to the pro‐oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II

TFIID is the main sequence‐specific DNA‐binding component of the RNA polymerase II (Pol II) transcriptional machinery. It is a multiprotein complex composed of the TATA‐binding protein (TBP) and TBP‐associated factors (TAF(II)s). Here we report the cloning and characterization of a novel human TBP‐a...

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Bibliographic Details
Published in:The EMBO journal 1996-09, Vol.15 (18), p.5022-5031
Main Authors: Bertolotti, A., Lutz, Y., Heard, D. J., Chambon, P., Tora, L.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biochemistry, Molecular Biology
DNA Polymerase II - metabolism
DNA, Single-Stranded - metabolism
Electrophoresis, Polyacrylamide Gel
HeLa Cells
Heterogeneous-Nuclear Ribonucleoproteins
Humans
Life Sciences
Molecular Sequence Data
Neoplasm Proteins - chemistry
Neoplasm Proteins - metabolism
Ribonucleoproteins - chemistry
Ribonucleoproteins - metabolism
RNA - metabolism
RNA, Heterogeneous Nuclear - metabolism
RNA-Binding Protein EWS
RNA-Binding Protein FUS
RNA-Binding Proteins - chemistry
RNA-Binding Proteins - metabolism
Sequence Alignment
TATA-Binding Protein Associated Factors
Transcription Factor TFIID
Transcription Factors - chemistry
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:TFIID is the main sequence‐specific DNA‐binding component of the RNA polymerase II (Pol II) transcriptional machinery. It is a multiprotein complex composed of the TATA‐binding protein (TBP) and TBP‐associated factors (TAF(II)s). Here we report the cloning and characterization of a novel human TBP‐associated factor, hTAF(II)68. It contains a consensus RNA‐binding domain (RNP‐CS) and binds not only RNA, but also single stranded (ss) DNA. hTAF(II)68 shares extensive sequence similarity with TLS/FUS and EWS, two human nuclear RNA‐binding pro‐oncoproteins which are products of genes commonly translocated in human sarcomas. Like hTAF(II)68, TLS/FUS is also associated with a sub‐population of TFIID complexes chromatographically separable from those containing hTAF(II)68. Therefore, these RNA and/or ssDNA‐binding proteins may play specific roles during transcription initiation at distinct promoters. Moreover, we demonstrate that hTAF(II)68 co‐purifies also with the human RNA polymerase II and can enter the preinitiation complex together with Pol II.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1996.tb00882.x