Deletion of rpoB reveals a second distinct transcription system in plastids of higher plants

The plastic genome in higher plants encodes subunits of an Escherichia coli-like RNA polymerase which initiates transcription of plastid genes from sequences resembling E. coli sigma 70-type promoters. By deleting the gene for the essential beta subunit of the tobacco E. coli-like RNA polymerase, we...

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Bibliographic Details
Published in:The EMBO journal 1996-06, Vol.15 (11), p.2802-2809
Main Authors: Allison, L.A, Simon, L.D, Maliga, P
Format: Article
Language:English
Subjects:
Base Sequence
chloroplast DNA
defects
deletions
DNA-directed RNA polymerase
DNA-Directed RNA Polymerases - genetics
Escherichia coli
gene expression
Gene Expression Regulation, Plant
genetic system genes
genomics
messenger RNA
Molecular Sequence Data
Nicotiana - genetics
Nicotiana tabacum
organelle mutations
Photosynthesis
photosynthetic genes
Plants, Toxic
plastids
Plastids - enzymology
promoter regions
Promoter Regions, Genetic
proteins
ribosomal DNA
ribosomal proteins
ribosomes
RNA, Ribosomal, 16S - genetics
rpob gene
Sequence Deletion
Sigma Factor - genetics
structural genes
thylakoids
transcription (genetics)
Transcription, Genetic
transgenic plants
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Summary:The plastic genome in higher plants encodes subunits of an Escherichia coli-like RNA polymerase which initiates transcription of plastid genes from sequences resembling E. coli sigma 70-type promoters. By deleting the gene for the essential beta subunit of the tobacco E. coli-like RNA polymerase, we have established the existence of a second plastic transcription system which does not utilize E. coli-like promoters. In contrast to the E. coli-like RNA polymerase, the novel transcription machinery preferentially transcribes genetic system genes rather than photosynthetic genes. Although the mutant plants are photosynthetically defective, transcription by this polymerase is sufficient for plastid maintenance and plant development.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1996.tb00640.x