Investigation of multipotent postnatal stem cells from human maxillary sinus membrane

Maxillary sinus membrane (MSM) elevation is a common surgical technique for increasing bone height in the posterior maxilla prior to dental implant placement. However, the biological nature of bone regeneration in MSM remains largely unidentified. In this study, MSM tissue was obtained from 16 indiv...

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Bibliographic Details
Published in:Scientific reports 2015-06, Vol.5 (1), p.11660-11660, Article 11660
Main Authors: Guo, JunBing, Weng, JunQuan, Rong, Qiong, Zhang, Xing, Zhu, ShuangXi, Huang, DaiYing, Li, Xiang, Chen, SongLing
Format: Article
Language:English
Subjects:
Adipocytes
Adipogenesis
Adolescent
Adult Stem Cells - cytology
Antigens
Bone growth
Bone Marrow Cells - cytology
CD29 antigen
CD44 antigen
Cell culture
Cell Separation
Cell surface
Chondrocytes
Chondrogenesis
Clone Cells
Colonies
Dental implants
Dental Pulp - cytology
Humans
Immunohistochemistry
Immunophenotyping
Maxilla
Maxillary sinus
Maxillary Sinus - cytology
Maxillary Sinus - diagnostic imaging
Mesenchyme
Multipotent Stem Cells - cytology
Multipotent Stem Cells - transplantation
Osteogenesis
Periodontal Ligament - cytology
Radiography
Regeneration
Sinus
Stem Cell Transplantation
Stem cells
Surface antigens
Surgery
Young Adult
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Summary:Maxillary sinus membrane (MSM) elevation is a common surgical technique for increasing bone height in the posterior maxilla prior to dental implant placement. However, the biological nature of bone regeneration in MSM remains largely unidentified. In this study, MSM tissue was obtained from 16 individuals during orthognathic surgery and used to isolate MSM stem cells (MSMSCs) by single-colony selection and STRO-1 cell sorting. The cell characteristics in terms of colony-forming ability, cell surface antigens, multi-differentiation potential and in vivo implantation were all evaluated. It was found that MSMSCs were of mesenchymal origin and positive for mesenchymal stem cell (MSC) markers such as STRO-1, CD146, CD29 and CD44; furthermore, under defined culture conditions, MSMSCs were able to form mineral deposits and differentiate into adipocytes and chondrocytes. When transplanted into immunocompromised rodents, MSMSCs showed the capacity to generate bone-like tissue and, importantly, maintain their MSC characteristics after in vivo implantation. These findings provide cellular and molecular evidence that MSM contains stem cells that show functional potential in bone regeneration for dental implant.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep11660