Effect of Variation in hemorheology between human and animal blood on the binding efficacy of vascular-targeted carriers

Animal models are extensively used to evaluate the in vivo functionality of novel drug delivery systems (DDS). However, many variations likely exist in vivo between the animals and human physiological environment that significantly alter results obtained with animal models relative to human system....

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Bibliographic Details
Published in:Scientific reports 2015-06, Vol.5 (1), p.11631-11631, Article 11631
Main Authors: Namdee, K., Carrasco-Teja, M., Fish, M. B., Charoenphol, P., Eniola-Adefeso, O.
Format: Article
Language:English
Subjects:
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639/925/350/354
Adult
Animal models
Animals
Blood flow
Blood Flow Velocity - physiology
Cell Adhesion
Cell Size
Cells, Cultured
Drug Carriers - chemistry
Drug Carriers - metabolism
Drug delivery
Drug Delivery Systems - methods
Endothelial cells
Erythrocytes
Erythrocytes - metabolism
Hemodynamics
Hemodynamics - physiology
Hemorheology - physiology
Human Umbilical Vein Endothelial Cells - metabolism
Humanities and Social Sciences
Humans
Inflammation
Leukocytes
Mice
Microspheres
Monte Carlo Method
multidisciplinary
Nanospheres - chemistry
Particle Size
Physiology
Rabbits
Science
Species Specificity
Swine
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Summary:Animal models are extensively used to evaluate the in vivo functionality of novel drug delivery systems (DDS). However, many variations likely exist in vivo between the animals and human physiological environment that significantly alter results obtained with animal models relative to human system. To date, it is not clear if the variation in hemorheology and hemodynamics between common animal and human models affect the functionality of DDS. This study investigates the role of hemorheology of humans and various animal models in dictating the binding efficiency of model vascular-targeted carriers (VTCs) to the wall in physiological blood flows. Specifically, the adhesion of sLe A -coated nano- and micro-spheres to inflamed endothelial cells monolayers were conducted via a parallel plate flow chamber assay with steady and disturbed red blood cells (RBCs)-in-buffer and whole blood flows of common animal models. Our results suggest that the ratio of carrier size to RBC size dictate particle binding in blood flow. Additionally, the presence of white blood cells affects the trend of particle adhesion depending on the animal species. Overall, this work sheds light on some deviation in VTC vascular wall interaction results obtained with in vivo animal experimentation from expected outcome and efficiency in vivo in human.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep11631