Expression of galectin‐1 in carcinoma‐associated fibroblasts promotes gastric cancer cell invasion through upregulation of integrin β1

Increased expression of galectin‐1 (Gal‐1) in carcinoma‐associated fibroblasts (CAFs) has been reported to correlate with progression and prognosis in many cancers. However, rarely have reports sought to determine whether high Gal‐1 expression in CAFs in gastric cancer is involved in the tumor proce...

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Bibliographic Details
Published in:Cancer science 2014-11, Vol.105 (11), p.1402-1410
Main Authors: He, Xu‐Jun, Tao, Hou‐Quan, Hu, Zhi‐Ming, Ma, Ying‐Yu, Xu, Ji, Wang, Hui‐Ju, Xia, Ying‐Jie, Li, Li, Fei, Bao‐Ying, Li, Ya‐Qing, Chen, Jian‐Zhong
Format: Article
Language:English
Subjects:
Biotechnology
Cancer therapies
Carcinoma‐associated fibroblasts
Cell adhesion & migration
Cell culture
Cell Line, Tumor
Cell migration
Enzymes
Female
Fibroblasts
Fibroblasts - metabolism
Fibroblasts - pathology
Galectin 1 - genetics
Galectin 1 - metabolism
Gal‐1
Gastric cancer
Gene Expression
Gene Expression Profiling
Growth factors
Humans
Integrin beta1 - genetics
Integrin beta1 - metabolism
integrin β1
invasion
Male
Medical prognosis
Metastasis
Neoplasm Grading
Neoplasm Invasiveness
Neoplasm Metastasis
Neoplasm Staging
Original
Proteins
siRNA
Smooth muscle
Stomach Neoplasms - genetics
Stomach Neoplasms - mortality
Stomach Neoplasms - pathology
Tumor Burden
Up-Regulation
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Summary:Increased expression of galectin‐1 (Gal‐1) in carcinoma‐associated fibroblasts (CAFs) has been reported to correlate with progression and prognosis in many cancers. However, rarely have reports sought to determine whether high Gal‐1 expression in CAFs in gastric cancer is involved in the tumor process, and the specific mechanism by which it promotes the evolution of gastric cancer is still unknown. In this study, we cultured gastric cancer CAFs, which showed strong expression of Gal‐1, and established a co‐culture system of CAFs with gastric cancer cells. Specific siRNA and in vitro migration and invasion assays were used to explore the effects of the interaction between Gal‐1 expression of CAFs and gastric cancer cells on cell migration and invasion. We found that the overexpression of Gal‐1 in CAFs enhanced gastric cancer cell migration and invasion, and these stimulatory effects could be blocked by specific siRNA which reduced the Gal‐1 expression level. A set of cancer invasion‐associated genes were then chosen to identify the possible mechanism of Gal‐1‐induced cell invasion. Among these genes, integrin β1 expression in cancer cells was considered to be associated with Gal‐1 expression. Pre‐blocking of the integrin β1 expression in gastric cancer cells with siRNA could interrupt the invasion‐promoting effect of CAFs with high Gal‐1 expression. Furthermore, immunohistochemical assay confirmed a positive correlation between Gal‐1 and integrin β1 expression. Our results showed that high expression of Gal‐1 in CAFs might facilitate gastric cancer cell migration and invasion by upregulating integrin β1 expression in gastric cancer. The aim of this study was to investigate whether high Gal‐1 expression in CAFs has any impact on the invasive or migratory behavior of gastric cancer cells and to explore its molecular mechanisms. And we found that that high expression of Gal‐1 in CAFs might facilitate gastric cancer cell migration and invasion by up‐regulating the integrin β1 expression in gastric cancer.
ISSN:1347-9032
1349-7006
DOI:10.1111/cas.12539