DNA methylation analysis of phenotype specific stratified Indian population

DNA methylation and its perturbations are an established attribute to a wide spectrum of phenotypic variations and disease conditions. Indian traditional system practices personalized medicine through indigenous concept of distinctly descriptive physiological, psychological and anatomical features k...

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Bibliographic Details
Published in:Journal of translational medicine 2015-05, Vol.13 (1), p.151-151, Article 151
Main Authors: Rotti, Harish, Mallya, Sandeep, Kabekkodu, Shama Prasada, Chakrabarty, Sanjiban, Bhale, Sameer, Bharadwaj, Ramachandra, Bhat, Balakrishna K, Dedge, Amrish P, Dhumal, Vikram Ram, Gangadharan, G G, Gopinath, Puthiya M, Govindaraj, Periyasamy, Joshi, Kalpana S, Kondaiah, Paturu, Nair, Sreekumaran, Nair, S N Venugopalan, Nayak, Jayakrishna, Prasanna, B V, Shintre, Pooja, Sule, Mayura, Thangaraj, Kumarasamy, Patwardhan, Bhushan, Valiathan, Marthanda Varma Sankaran, Satyamoorthy, Kapaettu
Format: Article
Language:English
Subjects:
Adult
Chromatography, High Pressure Liquid
CpG Islands
DNA - chemistry
DNA Methylation
Epigenesis, Genetic
Genomics
Humans
Immunoprecipitation
India
Male
Medicine, Ayurvedic
Oligonucleotide Array Sequence Analysis
Phenotype
Precision Medicine
Promoter Regions, Genetic
Sequence Analysis, DNA
Young Adult
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Summary:DNA methylation and its perturbations are an established attribute to a wide spectrum of phenotypic variations and disease conditions. Indian traditional system practices personalized medicine through indigenous concept of distinctly descriptive physiological, psychological and anatomical features known as prakriti. Here we attempted to establish DNA methylation differences in these three prakriti phenotypes. Following structured and objective measurement of 3416 subjects, whole blood DNA of 147 healthy male individuals belonging to defined prakriti (Vata, Pitta and Kapha) between the age group of 20-30years were subjected to methylated DNA immunoprecipitation (MeDIP) and microarray analysis. After data analysis, prakriti specific signatures were validated through bisulfite DNA sequencing. Differentially methylated regions in CpG islands and shores were significantly enriched in promoters/UTRs and gene body regions. Phenotypes characterized by higher metabolism (Pitta prakriti) in individuals showed distinct promoter (34) and gene body methylation (204), followed by Vata prakriti which correlates to motion showed DNA methylation in 52 promoters and 139 CpG islands and finally individuals with structural attributes (Kapha prakriti) with 23 and 19 promoters and CpG islands respectively. Bisulfite DNA sequencing of prakriti specific multiple CpG sites in promoters and 5'-UTR such as; LHX1 (Vata prakriti), SOX11 (Pitta prakriti) and CDH22 (Kapha prakriti) were validated. Kapha prakriti specific CDH22 5'-UTR CpG methylation was also found to be associated with higher body mass index (BMI). Differential DNA methylation signatures in three distinct prakriti phenotypes demonstrate the epigenetic basis of Indian traditional human classification which may have relevance to personalized medicine.
ISSN:1479-5876
1479-5876
DOI:10.1186/s12967-015-0506-0