Inhibitory action of novel hydrogen sulfide donors on bovine isolated posterior ciliary arteries

In the present study, we investigate the inhibitory effect of novel H2S donors, AP67 and AP72 on isolated bovine posterior ciliary arteries (PCAs) under conditions of tone induced by an adrenoceptor agonist. Furthermore, we examined the possible mechanisms underlying the AP67- and AP72-induced relax...

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Bibliographic Details
Published in:Experimental eye research 2015-05, Vol.134, p.73-79
Main Authors: Kulkarni-Chitnis, Madhura, Njie-Mbye, Ya Fatou, Mitchell, Leah, Robinson, Jenaye, Whiteman, Matthew, Wood, Mark E., Opere, Catherine A., Ohia, Sunny E.
Format: Article
Language:English
Subjects:
Adrenergic alpha-1 Receptor Agonists - pharmacology
Animals
Cattle
Ciliary Arteries - drug effects
Ciliary Arteries - physiology
Cystathionine beta-Synthase - antagonists & inhibitors
Cystathionine beta-Synthase - metabolism
Cystathionine gamma-Lyase - antagonists & inhibitors
Cystathionine gamma-Lyase - metabolism
Cystathionine β-synthase
Cystathionine γ-lyase
Dose-Response Relationship, Drug
Enzyme Inhibitors - pharmacology
Hydrogen sulfide
Hydrogen Sulfide - metabolism
Isometric Contraction - physiology
KATP channel
KATP Channels - metabolism
Muscle Relaxation - drug effects
Muscle, Smooth, Vascular - physiology
Nitric oxide
Nitric Oxide - metabolism
Ocular blood flow
Organophosphorus Compounds - pharmacology
Phenylephrine - pharmacology
Piperidines - pharmacology
Posterior ciliary artery
Pyrrolidines - pharmacology
Software Design
Vasoconstrictor Agents - pharmacology
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Summary:In the present study, we investigate the inhibitory effect of novel H2S donors, AP67 and AP72 on isolated bovine posterior ciliary arteries (PCAs) under conditions of tone induced by an adrenoceptor agonist. Furthermore, we examined the possible mechanisms underlying the AP67- and AP72-induced relaxations. Isolated bovine PCA were set up for measurement of isometric tension in organ baths containing oxygenated Krebs solution. The relaxant action of H2S donors was studied on phenylephrine-induced tone in the absence or presence of enzyme inhibitors for the following pathways: cyclooxygenase (COX); H2S; nitric oxide and the ATP-sensitive K+ (KATP) channel. The H2S donors, NaSH (1 nM – 10 μM), AP67 (1 nM – 10 μM) and AP72 (10 nM – 1 μM) elicited a concentration-dependent relaxation of phenylephrine-induced tone in isolated bovine PCA. While the COX inhibitor, flurbiprofen (3 μM) blocked significantly (p 
ISSN:0014-4835
1096-0007
DOI:10.1016/j.exer.2015.04.001