Motoneurons derived from induced pluripotent stem cells develop mature phenotypes typical of endogenous spinal motoneurons

Induced pluripotent cell-derived motoneurons (iPSCMNs) are sought for use in cell replacement therapies and treatment strategies for motoneuron diseases such as amyotrophic lateral sclerosis (ALS). However, much remains unknown about the physiological properties of iPSCMNs and how they compare with...

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Published in:The Journal of neuroscience 2015-01, Vol.35 (3), p.1291-1306
Main Authors: Toma, Jeremy S, Shettar, Basavaraj C, Chipman, Peter H, Pinto, Devanand M, Borowska, Joanna P, Ichida, Justin K, Fawcett, James P, Zhang, Ying, Eggan, Kevin, Rafuse, Victor F
Format: Article
Language:English
Subjects:
Animals
Axons - physiology
Chick Embryo
Induced Pluripotent Stem Cells - cytology
LIM-Homeodomain Proteins - metabolism
Mice
Motor Neurons - cytology
Muscle, Skeletal - cytology
Muscle, Skeletal - physiology
Neurogenesis - physiology
Neuromuscular Junction - cytology
Neuromuscular Junction - physiology
Phenotype
Proteomics
Transcription Factors - metabolism
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Summary:Induced pluripotent cell-derived motoneurons (iPSCMNs) are sought for use in cell replacement therapies and treatment strategies for motoneuron diseases such as amyotrophic lateral sclerosis (ALS). However, much remains unknown about the physiological properties of iPSCMNs and how they compare with endogenous spinal motoneurons or embryonic stem cell-derived motoneurons (ESCMNs). In the present study, we first used a proteomic approach and compared protein expression profiles between iPSCMNs and ESCMNs to show that
ISSN:0270-6474
1529-2401
DOI:10.1523/jneurosci.2126-14.2015