Anti-inflammatory mechanism of oxymatrine in dextran sulfate sodium-induced colitis of rats

AIM： To investigate the anti-inflammatory mechanism of oxymatrine in dextran sulfate sodium （DSS）-induced colitis of rats. METHODS： Acute colitis was induced by giving 2% DSS orally in drinking water for 8 d. Twenty-six male rats were randomized into oxymatrine-treated group （group A, 10 rats）, DSS...

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Published in:World journal of gastroenterology : WJG 2005-08, Vol.11 (31), p.4912-4915
Main Authors: Zheng, Ping, Niu, Feng-Li, Liu, Wen-Zhong, Shi, Yao, Lu, Lun-Gen
Format: Article
Language:English
Subjects:
Alkaloids - pharmacology
Animals
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Brief Reports
Colitis - chemically induced
Colitis - pathology
Colitis - prevention & control
Dextran Sulfate - toxicity
Disease Models, Animal
Interleukin-6 - blood
Male
NF-kappa B - metabolism
Quinolizines
Rats
Rats, Sprague-Dawley
Tumor Necrosis Factor-alpha - metabolism
动物实验
右旋糖苷
大肠炎
抗炎机制
氧化苦参碱
硫酸钠
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Summary:AIM： To investigate the anti-inflammatory mechanism of oxymatrine in dextran sulfate sodium （DSS）-induced colitis of rats. METHODS： Acute colitis was induced by giving 2% DSS orally in drinking water for 8 d. Twenty-six male rats were randomized into oxymatrine-treated group （group A, 10 rats）, DSS control （group B, 10 rats） and normal control （group C, 6 rats）. The rats in group A were injected muscularly with oxymatrine at the dosage of 63 mg/（kg·d） from d 1 to 11 and drank 2% DSS solution from d 4 to 11. The rats in group B were treated with 0.9% saline in an equal volume as group A and drank 2% DSS solution from d 4 to 11. The rats in group C were treated with 0.9% saline as group B from d 1 to 11 and drank water normally. Diarrhea and bloody stool as well as colonic histology were observed. The levels of serum tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） were determined by ELISA, and nuclear factor-κB （NF-κB） activity and the expression of inter-cellular adhesion molecule-1 （ICAM-1） in colonic mucosa were detected by immunohistochemistry method. RESULTS： Compared with DSS control group, the inflammatory symptoms and histological damages of colonic mucosa in oxymatrine-treated group were significantly improved, the serum levels of TNF-α, IL-6, and the expression of NF-κB, ICAM-1 in colonic mucosa were significantly reduced. CONCLUSION： The fact that oxymatrine can reduce the serum levels of TNF-α, IL-6, and the expression of NF-κB and ICAM-1 in colonic mucosa in DSS-induced colitis of rats indicates that oxymatrine may ameliorate the colonic inflammation and thus alleviate diarrhea and bloody stool.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v11.i31.4912