Apoptosis of pancreatic cancer BXPC-3 cells induced by indole-3-acetic acid in combination with horseradish peroxidase

AIM： To explore the mechanisms underlying the apoptosis of human pancreatic cancer BXPC-3 cells induced by indole-3-acetic acid （IAA） in combination with horseradish peroxidase （HRP）. METHODS： BXPC-3 cells derived from human pancreatic cancer were exposed to 40 or 80 μmol/L IAA and 1.2 μg/mL HRP at...

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Published in:World journal of gastroenterology : WJG 2005-08, Vol.11 (29), p.4519-4523
Main Authors: Huang, Chen, Liu, Li-Ying, Song, Tu-Sheng, Ni, Lei, Yang, Ling, Hu, Xiao-Yan, Hu, Jing-Song, Song, Li-Ping, Luo, Yu, Si, Lu-Sheng
Format: Article
Language:English
Subjects:
Apoptosis - drug effects
Basic Research
BXPC-3细胞
Cell Line, Tumor
Drug Therapy, Combination
Horseradish Peroxidase - pharmacology
Humans
Indoleacetic Acids - pharmacology
Pancreatic Neoplasms - drug therapy
Plant Growth Regulators - pharmacology
吲哚-3-乙酸
胰腺癌
过氧化酶化合物
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Summary:AIM： To explore the mechanisms underlying the apoptosis of human pancreatic cancer BXPC-3 cells induced by indole-3-acetic acid （IAA） in combination with horseradish peroxidase （HRP）. METHODS： BXPC-3 cells derived from human pancreatic cancer were exposed to 40 or 80 μmol/L IAA and 1.2 μg/mL HRP at different times. Then, Mn- assay was used to detect the cell proliferation. Flow cytometry was performed to analyze cell cycle. Terminal deoxynucleotidyl transferasemediated dUTP nick end labeling assay was used to detect apoptosis. 2,7-Dichlorofluorescin diacetate uptake was measured by confocal microscopy to determine free radicals. Level of malondialdehyde （MDA） and activity of superoxide dismutase （SOD） were measured by biochemical methods. RESULTS： IAA/HRP initiated growth inhibition of BXPC-3 cells in a dose- and time-dependent manner. Flow cytometry revealed that the cells treated for 48 h were arrested at G1/G0. After exposure to 80 μmol/L IAA plus 1.2 μg/mL HRP for 72 h, the apoptosis rate increased to 72.5‰, which was nine times that of control. Content of MDA and activity of SOD increased respectively after treatment compared to control. Meanwhile, IAA/HRP stimulated the formation of free radicals. CONCLUSION： The combination of IAA and HRP can inhibit the growth of human pancreatic cancer BXPC-3 cells in vitro by inducing apoptosis.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v11.i29.4519