N17 Modifies Mutant Huntingtin Nuclear Pathogenesis and Severity of Disease in HD BAC Transgenic Mice

The nucleus is a critical subcellular compartment for the pathogenesis of polyglutamine disorders, including Huntington’s disease (HD). Recent studies suggest the first 17-amino-acid domain (N17) of mutant huntingtin (mHTT) mediates its nuclear exclusion in cultured cells. Here, we test whether N17...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 2015-02, Vol.85 (4), p.726-741
Main Authors: Gu, Xiaofeng, Cantle, Jeffrey P., Greiner, Erin R., Lee, C.Y. Daniel, Barth, Albert M., Gao, Fuying, Park, Chang Sin, Zhang, Zhiqiang, Sandoval-Miller, Susana, Zhang, Richard L., Diamond, Marc, Mody, Istvan, Coppola, Giovanni, Yang, X. William
Format: Article
Language:English
Subjects:
Age Factors
Animals
Artificial chromosomes
Brain - metabolism
Brain - pathology
Brain research
Cell Nucleolus - metabolism
Cell Nucleolus - pathology
Data analysis
Disease
Disease Models, Animal
Female
Gene Expression Regulation - genetics
Genetic engineering
HEK293 Cells - ultrastructure
Humans
Huntingtin Protein
Huntington Disease - complications
Huntington Disease - genetics
Huntington Disease - pathology
Locomotion - genetics
Male
Mice
Mice, Transgenic
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Neurodegeneration
Neurons - metabolism
Neurons - pathology
Nuclear Proteins - genetics
Organ Size - genetics
Pathogenesis
Phenotype
Phosphorylation
Protein Structure, Tertiary - genetics
Proteins
Rodents
Studies
Subcellular Fractions - metabolism
Subcellular Fractions - pathology
Transgenic animals
Trinucleotide Repeat Expansion - genetics
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Summary:The nucleus is a critical subcellular compartment for the pathogenesis of polyglutamine disorders, including Huntington’s disease (HD). Recent studies suggest the first 17-amino-acid domain (N17) of mutant huntingtin (mHTT) mediates its nuclear exclusion in cultured cells. Here, we test whether N17 could be a molecular determinant of nuclear mHTT pathogenesis in vivo. BAC transgenic mice expressing mHTT lacking the N17 domain (BACHD-ΔN17) show dramatically accelerated mHTT pathology exclusively in the nucleus, which is associated with HD-like transcriptionopathy. Interestingly, BACHD-ΔN17 mice manifest more overt disease-like phenotypes than the original BACHD mice, including body weight loss, movement deficits, robust striatal neuron loss, and neuroinflammation. Mechanistically, N17 is necessary for nuclear exclusion of small mHTT fragments that are part of nuclear pathology in HD. Together, our study suggests that N17 modifies nuclear pathogenesis and disease severity in HD mice by regulating subcellular localization of known nuclear pathogenic mHTT species. •Mutant huntingtin lacking the N17 domain accelerates nuclear pathogenesis in mice•BACHD-ΔN17 mice exhibit progressive overt motor deficits and neurological decline•BACHD-ΔN17 mice show HD-like striatal neuron loss and neuroinflammation•N17 mediates cytoplasmic targeting of known nuclear pathogenic mHTT fragments Huntington’s disease (HD) mice expressing mutant huntingtin lacking the N17 domain manifest overt motor deficits and striatal neurodegeneration that mimic those seen in HD patients. The study shows a pivotal role for N17 in modifying disease pathogenesis in the nucleus.
ISSN:0896-6273
1097-4199
DOI:10.1016/j.neuron.2015.01.008