Soft tissue ossification and condylar cartilage degeneration following TMJ disc perforation in a rabbit pilot study

Summary Objective There are limited clinical treatments for temporomandibular joint (TMJ) pathologies, including degenerative disease, disc perforation and heterotopic ossification (HO). One barrier hindering the development of new therapies is that animal models recapitulating TMJ diseases are poor...

Full description

Saved in:
Bibliographic Details
Published in:Osteoarthritis and cartilage 2015-04, Vol.23 (4), p.629-639
Main Authors: Embree, M.C, Iwaoka, G.M, Kong, D, Martin, B.N, Patel, R.K, Lee, A.H, Nathan, J.M, Eisig, S.B, Safarov, A, Koslovsky, D.A, Koch, A, Romanov, A, Mao, J.J
Format: Article
Language:English
Subjects:
Animals
Biopsy, Needle
Cartilage Diseases - etiology
Cartilage Diseases - pathology
Cartilage, Articular - pathology
Cells, Cultured
Cost-Benefit Analysis
Disease Models, Animal
Fibrocartilage
Fibrocartilage - pathology
Heterotopic ossification
Mandibular Condyle - pathology
Ossification, Heterotopic - etiology
Ossification, Heterotopic - pathology
Osteoarthritis - etiology
Osteoarthritis - pathology
Osteogenesis
Pilot Projects
Rabbits
Rheumatology
Surgical osteoarthritis model
Temporomandibular Joint Disc - injuries
Temporomandibular Joint Disc - pathology
Temporomandibular Joint Disc - surgery
TMJ
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Objective There are limited clinical treatments for temporomandibular joint (TMJ) pathologies, including degenerative disease, disc perforation and heterotopic ossification (HO). One barrier hindering the development of new therapies is that animal models recapitulating TMJ diseases are poorly established. The objective of this study was to develop an animal model for TMJ cartilage degeneration and disc pathology, including disc perforation and soft tissue HO. Methods New Zealand white rabbits (n = 9 rabbits) underwent unilateral TMJ disc perforation surgery and sham surgery on the contralateral side. A 2.5 mm defect was created using a punch biopsy in rabbit TMJ disc. The TMJ condyles and discs were evaluated macroscopically and histologically after 4, 8 and 12 weeks. Condyles were blindly scored by four independent observers using OARSI recommendations for macroscopic and histopathological scoring of osteoarthritis (OA) in rabbit tissues. Results Histological evidence of TMJ condylar cartilage degeneration was apparent in experimental condyles following disc perforation relative to sham controls after 4 and 8 weeks, including surface fissures and loss of Safranin O staining. At 12 weeks, OARSI scores indicated experimental condylar cartilage erosion into the subchondral bone. Most strikingly, HO occurred within the TMJ disc upon perforation injury in six rabbits after 8 and 12 weeks. Conclusion We report for the first time a rabbit TMJ injury model that demonstrates condylar cartilage degeneration and disc ossification, which is indispensible for testing the efficacy of potential TMJ therapies.
ISSN:1063-4584
1522-9653
DOI:10.1016/j.joca.2014.12.015