Contrast-enhanced ultrasonography for the evaluation of liver fibrosis after biliary obstruction

AIM:To investigate perfusion change in contrastenhanced ultrasonography(CEUS) to evaluate liver fibrosis based on biliary obstruction using an animal model.METHODS:New Zealand white rabbits(3-4 kg) underwent bile duct ligation to form a biliary obstruction model.We performed liver CEUS and laborator...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2015-03, Vol.21 (9), p.2614-2621
Main Authors: Shin, Hyun Joo, Chang, Eun Young, Lee, Hye Sun, Hong, Jung Hwa, Park, Gyuri, Kim, Hyun Gi, Kim, Myung-Joon, Lee, Mi-Jung
Format: Article
Language:English
Subjects:
Animals
Basic Study
Cholestasis - complications
Contrast Media
enhanced
Feasibility Studies
Liver - blood supply
Liver - diagnostic imaging
Liver Circulation
Liver Cirrhosis, Experimental - diagnostic imaging
Liver Cirrhosis, Experimental - etiology
Liver Cirrhosis, Experimental - physiopathology
Male
Microcirculation
Perfusion Imaging - methods
Phospholipids
Predictive Value of Tests
Rabbits
Reproducibility of Results
Severity of Illness Index
Sulfur Hexafluoride
Time Factors
Ultrasonography
Ultrasonography
Contrast
ultrasound
Liver
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Summary:AIM:To investigate perfusion change in contrastenhanced ultrasonography(CEUS) to evaluate liver fibrosis based on biliary obstruction using an animal model.METHODS:New Zealand white rabbits(3-4 kg) underwent bile duct ligation to form a biliary obstruction model.We performed liver CEUS and laboratory tests on the day before the operation(day 0) and every 7 postoperative days until the rabbits were sacrificed.After CEUS,signal intensity of liver parenchyma with a time-intensity curve was analyzed.Perfusion parameters were automatically calculated from regionof-interests,including peak signal intensity,mean transit time,area under the curve and time to peak.Histological grades of liver fibrosis were assessed according to the Metavir score system immediately after sacrifice.Generalized estimating equations were used to analyze the association between liver fibrosis grades and perfusion parameters for statistical analysis.The perfusion parameters were measured on the last day and the difference between day 0 and the last day were evaluated.RESULTS:From the nine rabbits,histological grades of liver fibrosis were grade 1 in one rabbit,grade 2 and 3 in three rabbits each,and grade 4 in two rabbits.Among the four CEUS parameters,only the peak signal intensity measured on the last day demonstrated a significant association with liver fibrosis grades(OR =1.392,95%CI:1.114-1.741,P =0.004).The difference in peak signal intensity between day 0 and the last dayalso demonstrated an association with liver fibrosis(OR =1.191,95%CI:0.999-1.419,P =0.051).The other parameters tested,including mean transit time,area under the curve,and time to peak,showed no significant correlation with liver fibrosis grades.CONCLUSION:This animal study demonstrates that CEUS can be used to evaluate liver fibrosis from biliary obstruction using peak signal intensity as a parameter.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v21.i9.2614