Tubulin Synthesis on Polysomes Isolated from Brain and Leg Muscle of Embryonic Chick

A protein-synthesizing system has been established in vitro, in which tubulin is synthesized on polysomes isolated from brain and leg muscle of embryonic chick. The tubulin synthesized in vitro is characterized by (i) its electrophoretic mobility on sodium dodecyl sulfate-acrylamide gels (brain: 55,...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1974-11, Vol.71 (11), p.4278-4282
Main Authors: Jorgensen, Annelise O., Heywood, Stuart M.
Format: Article
Language:English
Subjects:
Actins
Animals
Biological Sciences: Biochemistry
Brain - ultrastructure
Cell Fractionation
Cell-Free System
Chick Embryo
Chromatography, Ion Exchange
Depolymerization
Electrophoresis, Polyacrylamide Gel
Gels
Legs
Microtubules
Molecular Weight
Monomers
Muscles - ultrastructure
Nerve Tissue Proteins - biosynthesis
Polymerization
Polymers
Polyribosomes
Polyribosomes - metabolism
Sodium
Sulfur Radioisotopes
Tritium
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Summary:A protein-synthesizing system has been established in vitro, in which tubulin is synthesized on polysomes isolated from brain and leg muscle of embryonic chick. The tubulin synthesized in vitro is characterized by (i) its electrophoretic mobility on sodium dodecyl sulfate-acrylamide gels (brain: 55,000 daltons; leg muscle: 53,000 daltons), and (ii) its ability to function as a microtubular subunit, as judged by its specific ability to participate in at least two polymerization and depolymerization steps in the microtubule assembly system in vitro.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.71.11.4278