Development and pyrosequencing analysis of an in-vitro oral biofilm model

Dental caries and periodontal disease are the commonest bacterial diseases of man and can result in tooth loss. The principal method of prevention is the mechanical removal of dental plaque augmented by active agents incorporated into toothpastes and mouthrinses. In-vitro assays that include complex...

Full description

Saved in:
Bibliographic Details
Published in:BMC microbiology 2015-02, Vol.15 (1), p.24-24, Article 24
Main Authors: Kistler, James O, Pesaro, Manuel, Wade, William G
Format: Article
Language:English
Subjects:
Analysis
Analysis of Variance
Bacterial infections
Biofilms - growth & development
Culture Media
Dental Plaque - microbiology
Dentifrices
Fusobacterium nucleatum - classification
Fusobacterium nucleatum - genetics
Fusobacterium nucleatum - growth & development
High-Throughput Nucleotide Sequencing
Humans
Microbial Consortia - genetics
Microbiota (Symbiotic organisms)
Peptostreptococcus - classification
Peptostreptococcus - genetics
Peptostreptococcus - growth & development
Phylogeny
Prevotella - classification
Prevotella - genetics
Prevotella - growth & development
Reproducibility of Results
RNA, Ribosomal, 16S - genetics
Saliva - microbiology
Streptococcus constellatus - classification
Streptococcus constellatus - genetics
Streptococcus constellatus - growth & development
Time Factors
Veillonella - classification
Veillonella - genetics
Veillonella - growth & development
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Dental caries and periodontal disease are the commonest bacterial diseases of man and can result in tooth loss. The principal method of prevention is the mechanical removal of dental plaque augmented by active agents incorporated into toothpastes and mouthrinses. In-vitro assays that include complex oral bacterial biofilms are required to accurately predict the efficacy of novel active agents in vivo. The aim of this study was to develop an oral biofilm model using the Calgary biofilm device (CBD) seeded with a natural saliva inoculum and analysed by next generation sequencing. The specific objectives were to determine the reproducibility and stability of the model by comparing the composition of the biofilms over time derived from (i) the same volunteers at different time points, and (ii) different panels of volunteers. Pyrosequencing yielded 280,093 sequences with a mean length of 432 bases after filtering. A mean of 320 and 250 OTUs were detected in pooled saliva and biofilm samples, respectively. Principal coordinates analysis (PCoA) plots based on community membership and structure showed that replicate biofilm samples were highly similar and clustered together. In addition, there were no significant differences between biofilms derived from the same panel at different times using analysis of molecular variance (AMOVA). There were significant differences between biofilms from different panels (AMOVA, P 
ISSN:1471-2180
1471-2180
DOI:10.1186/s12866-015-0364-1