Dynamic assessment of cell viability, proliferation and migration using real time cell analyzer system (RTCA)

Cell viability and cell migration capacities are critical parameters for cell culture-related studies. It is essential to monitor the dynamic changes of cell properties under various co-culture conditions to our better understanding of their behaviours and characteristics. The real time cell analyze...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 2015-03, Vol.67 (2), p.379-386
Main Authors: Roshan Moniri, Mani, Young, Ada, Reinheimer, Kelsey, Rayat, Jarrett, Dai, Long-Jun, Warnock, Garth L.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedicine
Biotechnology
Cancer therapies
Cell adhesion & migration
Cell culture
Cell cycle
Cell migration
Cell viability
Chemistry
Chemistry and Materials Science
Cytotoxicity
Electrodes
Mesenchymal stem cells
Method in Cell Science
Microscopy
Pancreatic cancer
Real time
Stem cells
Wound healing
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Summary:Cell viability and cell migration capacities are critical parameters for cell culture-related studies. It is essential to monitor the dynamic changes of cell properties under various co-culture conditions to our better understanding of their behaviours and characteristics. The real time cell analyzer (RTCA, xCELLigence, Roche) is an impedance-based technology that can be used for label-free and real-time monitoring of cell properties, such as cell adherence, proliferation, migration and cytotoxicity. The practicality of this system has been proven in our recent cancer studies. In the present method, we intend to use co-cultures of pancreatic cancer cells (HP62) and mesenchymal stem cells to describe in detail, the procedures and benefits of RTCA.
ISSN:0920-9069
1573-0778
DOI:10.1007/s10616-014-9692-5