Helicobacter pylori infection in relation to E-cadherin gene promoter polymorphism and hypermethylation in sporadic gastric carcinomas

AIM： To study Helicobacter pylori （H pylori） infection in relation to E-cadherin （E-cad） promoter polymorphism and hypermethylation in GCs. METHODS： Specimens were taken from representative cancerous lesions and adjacent non-cancerous epithelia of 67 resected GCs. Hpyloriwas detected by real-time PC...

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Published in:World journal of gastroenterology : WJG 2005-09, Vol.11 (33), p.5174-5179
Main Authors: Liu, Yao-Chi, Shen, Chen-Yang, Wu, Hurng-Sheng, Chan, De-Chuan, Chen, Cheng-Jueng, Yu, Jyh-Cherng, Yu, Cheng-Ping, Harn, Horng-Jyh, Shyu, Rong-Yaun, Shih, Yu-Lueng, Hsieh, Chung-Bao, Hsu, Huan-Mieng
Format: Article
Language:English
Subjects:
Cadherins - genetics
Carcinoma - complications
DNA Methylation
E-钙粘素
Helicobacter Infections - complications
Helicobacter Pylori
Humans
Polymorphism, Genetic
Promoter Regions, Genetic
Stomach Neoplasms - complications
基因多态性
幽门螺杆菌
细菌感染
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Summary:AIM： To study Helicobacter pylori （H pylori） infection in relation to E-cadherin （E-cad） promoter polymorphism and hypermethylation in GCs. METHODS： Specimens were taken from representative cancerous lesions and adjacent non-cancerous epithelia of 67 resected GCs. Hpyloriwas detected by real-time PCR of the cagA gene from non-neoplastic epithelium. E-cadpromoter polymorphism and hypermethylation were determined by restriction fragment length polymorphism analysis and methylation-specific PCR, respectively. Expression of E-cadprotein was determined by immunohistochemistry. RESULTS： Hpyloriwas found in 57% of patients with GC. Hpyloriinfection was more frequently found in tumors with the -160C/C genotype than those with the -160C/A and -160A/A genotypes （74% vs 47%, P = 0.02）. Hpylori infection was associated with E-cad methylation in nonneoplastic epithelium; however, no significant difference in H pylori was observed between methylated and unmethylated cancerous lesions. CONCLUSION： Patients with the -160C/C genotype might require H pyloriinfection to promote the inactivation of CDH1, suggesting that Hpyloriinfection might affect GC in an initial stage because polymorphism is germ line. Mechanism of hypermethylation of CDH1 promoter in GC is complex, and Hpyloriinfection might affect it in an initial stage.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v11.i33.5174