Rapid inertial solution exchange for enrichment and flow cytometric detection of microvesicles

Exosomes, nanosized membrane-bound vesicles released by cells, play roles in cell signaling, immunology, virology, and oncology. Their study, however, has been hampered by difficulty in isolation and quantification due to their size and the complexity of biological samples. Conventional approaches t...

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Bibliographic Details
Published in:Biomicrofluidics 2015-01, Vol.9 (1), p.014112-014112
Main Authors: Dudani, Jaideep S, Gossett, Daniel R, Tse, Henry T K, Lamm, Robert J, Kulkarni, Rajan P, Carlo, Dino Di
Format: Article
Language:English
Subjects:
Beads
Biological properties
Fluorescence
Immunology
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Summary:Exosomes, nanosized membrane-bound vesicles released by cells, play roles in cell signaling, immunology, virology, and oncology. Their study, however, has been hampered by difficulty in isolation and quantification due to their size and the complexity of biological samples. Conventional approaches to improved isolation require specialized equipment and extensive sample preparation time. Therefore, isolation and detection methods of exosomes will benefit biological and clinical studies. Here, we report a microfluidic platform for inline exosome isolation and fluorescent detection using inertial manipulation of antibody-coated exosome capture beads from biological fluids.
ISSN:1932-1058
1932-1058
DOI:10.1063/1.4907807