Cell Volume Fluctuations in MDCK Monolayers

Cells moving collectively in tissues constitute a form of active matter, in which collective motion depends strongly on driven fluctuations at the single-cell scale. Fluctuations in cell area and number density are often seen in monolayers, yet their role in collective migration is not known. Here w...

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Bibliographic Details
Published in:Biophysical journal 2015-01, Vol.108 (2), p.247-250
Main Authors: Zehnder, Steven M., Suaris, Melanie, Bellaire, Madisonclaire M., Angelini, Thomas E.
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Biophysical Letter
Biophysics
Cell Movement
Cell Shape
Cell Size
Cells
Cytoskeleton
Dogs
Madin Darby Canine Kidney Cells
Tissues
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Summary:Cells moving collectively in tissues constitute a form of active matter, in which collective motion depends strongly on driven fluctuations at the single-cell scale. Fluctuations in cell area and number density are often seen in monolayers, yet their role in collective migration is not known. Here we study density fluctuations at the single- and multicell level, finding that single-cell volumes oscillate with a timescale of 4 h and an amplitude of 20%; the timescale and amplitude are found to depend on cytoskeletal activity. At the multicellular scale, density fluctuations violate the central limit theorem, highlighting the role of nonequilibrium driving forces in multicellular density fluctuations.
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2014.11.1856