Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor of Macrophage

Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homolo...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2014, Vol.76(12), pp.1641-1645
Main Authors: TOMURA, Shintaro, UCHIDA, Mona, YONEZAWA, Tomohiro, KOBAYASHI, Masato, BONKOBARA, Makoto, ARAI, Satoko, MIYAZAKI, Toru, TAMAHARA, Satoshi, MATSUKI, Naoaki
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
apoptosis inhibitor of macrophage (AIM)
Base Sequence
canine
CD36
CD5L
Cloning, Molecular
DNA Primers - genetics
DNA, Complementary - genetics
Dogs - genetics
Dogs - metabolism
Gene Expression Profiling
Internal Medicine
Liver - metabolism
Lung - metabolism
Lymph Nodes - metabolism
Molecular Sequence Data
Receptors, Scavenger - genetics
Receptors, Scavenger - metabolism
scavenger receptor cysteine-rich (SRCR) superfamily
Sequence Alignment
Sequence Analysis, DNA - veterinary
Sequence Homology
Spleen - metabolism
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Summary:Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homology with those of the human, mouse and rat. Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages. Moreover, AIM was highly expressed in one of the canine histiocytic sarcoma cell lines. CD36, the receptor of AIM, was also expressed in various tissues and these cell lines. These findings are useful to reveal the actual functions of canine AIM.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.14-0166