Rab geranylgeranyl transferase β subunit is essential for male fertility and tip growth in Arabidopsis

Rab proteins, key players in vesicular transport in all eukaryotic cells, are post-translationally modified by lipid moieties. Two geranylgeranyl groups are attached to the Rab protein by the heterodimeric enzyme Rab geranylgeranyl transferase (RGT) αβ. Partial impairment in this enzyme activity in...

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Bibliographic Details
Published in:Journal of experimental botany 2015-01, Vol.66 (1), p.213-224
Main Authors: Gutkowska, Malgorzata, Wnuk, Marta, Nowakowska, Julita, Lichocka, Malgorzata, Stronkowski, Michal M., Swiezewska, Ewa
Format: Article
Language:English
Subjects:
Alkyl and Aryl Transferases - chemistry
Alkyl and Aryl Transferases - genetics
Alkyl and Aryl Transferases - metabolism
Amino Acid Sequence
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis Proteins - chemistry
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Base Sequence
Fertility - genetics
Flowers - anatomy & histology
Flowers - genetics
Flowers - growth & development
Molecular Sequence Data
Mutation
Pollen - metabolism
Pollen Tube - growth & development
Reproduction
RESEARCH PAPER
Transferases - chemistry
Transferases - genetics
Transferases - metabolism
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Summary:Rab proteins, key players in vesicular transport in all eukaryotic cells, are post-translationally modified by lipid moieties. Two geranylgeranyl groups are attached to the Rab protein by the heterodimeric enzyme Rab geranylgeranyl transferase (RGT) αβ. Partial impairment in this enzyme activity in Arabidopsis, by disruption of the AtRGTB1 gene, is known to influence plant stature and disturb gravitropic and light responses. Here it is shown that mutations in each of the RGTB genes cause a tip growth defect, visible as root hair and pollen tube deformations. Moreover, FM 1–43 styryl dye endocytosis and recycling are affected in the mutant root hairs. Finally, it is demonstrated that the double mutant, with both AtRGTB genes disrupted, is non-viable due to absolute male sterility. Doubly mutated pollen is shrunken, has an abnormal exine structure, and shows strong disorganization of internal membranes, particularly of the endoplasmic reticulum system.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/eru412