Evaluation of the Carba NP test for carbapenemase detection

The Carba NP test was evaluated against a panel of 61 carbapenemase-producing bacterial species (15 producing class A carbapenemases, 15 producing class D carbapenemases, and 31 producing metallo-β-lactamases) and against 111 isolates with non-wild-type carbapenem susceptibility but not producing ca...

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Bibliographic Details
Published in:Antimicrobial agents and chemotherapy 2014-12, Vol.58 (12), p.7553-7556
Main Authors: Osterblad, Monica, Hakanen, Antti J, Jalava, Jari
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents
Anti-Bacterial Agents - metabolism
Anti-Bacterial Agents - pharmacology
Bacterial Proteins
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
beta-Lactamases
beta-Lactamases - genetics
beta-Lactamases - metabolism
Citrobacter freundii - drug effects
Citrobacter freundii - enzymology
Citrobacter freundii - genetics
Enterobacter aerogenes - drug effects
Enterobacter aerogenes - enzymology
Enterobacter aerogenes - genetics
Enterobacter cloacae - drug effects
Enterobacter cloacae - enzymology
Enterobacter cloacae - genetics
Enzyme Assays
Enzyme Assays - standards
Escherichia coli - drug effects
Escherichia coli - enzymology
Escherichia coli - genetics
Gene Expression
Hydrolysis
Imipenem
Imipenem - metabolism
Imipenem - pharmacology
Klebsiella oxytoca - drug effects
Klebsiella oxytoca - enzymology
Klebsiella oxytoca - genetics
Klebsiella pneumoniae - drug effects
Klebsiella pneumoniae - enzymology
Klebsiella pneumoniae - genetics
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - enzymology
Pseudomonas aeruginosa - genetics
Susceptibility
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Summary:The Carba NP test was evaluated against a panel of 61 carbapenemase-producing bacterial species (15 producing class A carbapenemases, 15 producing class D carbapenemases, and 31 producing metallo-β-lactamases) and against 111 isolates with non-wild-type carbapenem susceptibility but not producing carbapenemase. Carbapenemase production was verified by PCR and UV-spectrophotometric measurement of imipenem hydrolysis. No false positives were seen, but there were consistent problems with the detection of OXA-48-like enzymes and also some rarer class A enzymes.
ISSN:0066-4804
1098-6596
DOI:10.1128/aac.02761-13