Molecular features of community-associated extended-spectrum-β-lactamase-producing Escherichia coli strains in the United States

We characterized 30 community-associated extended-spectrum-β-lactamase-producing Escherichia coli isolates collected from five hospitals in the United States. Nineteen sequence types were identified. All sequence type 131 (ST131) isolates had the fimH30 allele. IncFII-FIA-FIB was the most common rep...

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Bibliographic Details
Published in:Antimicrobial agents and chemotherapy 2014-11, Vol.58 (11), p.6953-6957
Main Authors: Hu, Fupin, O'Hara, Jessica A, Rivera, Jesabel I, Doi, Yohei
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents
Anti-Bacterial Agents - pharmacology
Bacterial Typing Techniques
Base Sequence
beta-Lactamases
beta-Lactamases - biosynthesis
beta-Lactamases - genetics
Cohort Studies
Community-Acquired Infections - epidemiology
Drug Resistance, Multiple, Bacterial
Drug Resistance, Multiple, Bacterial - genetics
Epidemiology and Surveillance
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - genetics
Escherichia coli Infections
Escherichia coli Infections - epidemiology
Escherichia coli Infections - microbiology
Humans
Microbial Sensitivity Tests
Molecular Epidemiology
Multilocus Sequence Typing
Sequence Analysis, DNA
United States - epidemiology
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Summary:We characterized 30 community-associated extended-spectrum-β-lactamase-producing Escherichia coli isolates collected from five hospitals in the United States. Nineteen sequence types were identified. All sequence type 131 (ST131) isolates had the fimH30 allele. IncFII-FIA-FIB was the most common replicon type among the blaCTX-M-carrying plasmids, followed by IncFII-FIA and IncA/C. Restriction analysis of the IncFII-FIA-FIB and IncFII-FIA plasmids yielded related profiles for plasmids originating from different hospitals. The plasmids containing blaCTX-M or blaSHV were stably maintained after serial passages.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.03321-14