Glytan decreases portal pressure via mesentery vasoconstriction in portal hypertensive rats

AIM: To investigate the effects of Glytan on splanchnic hemodynamics and its reduction of portal pressure in portal hypertensive rats.METHODS:Glytan(Ganluotong in Chinese),is composed of salvianolic acid B and diammonium glycyrrhizinate.Portal hypertension(PHT)was induced in the rats by common bile...

Full description

Saved in:
Bibliographic Details
Published in:World journal of gastroenterology : WJG 2014-11, Vol.20 (44), p.16674-16682, Article 16674
Main Authors: Du, Qing-Hong, Han, Lin, Jiang, Jun-Jie, Xu, Ya, Li, Wei-Hong, Li, Peng-Tao, Wang, Xin-Yue, Jia, Xu
Format: Article
Language:English
Subjects:
Animals
Benzofurans - pharmacology
Blood Flow Velocity
Disease Models, Animal
Drugs, Chinese Herbal - pharmacology
Endothelin B Receptor Antagonists - pharmacology
Endothelin-1 - blood
G-Protein-Coupled Receptor Kinase 2 - antagonists & inhibitors
G-Protein-Coupled Receptor Kinase 2 - metabolism
Glycyrrhetinic Acid - analogs & derivatives
Glycyrrhetinic Acid - pharmacology
Glytan
Hemodynam-ics
hypertension
Hypertension, Portal - blood
Hypertension, Portal - drug therapy
Hypertension, Portal - enzymology
Hypertension, Portal - physiopathology
Male
Mesentery
Mesentery - blood supply
Mesentery - drug effects
Mesentery - enzymology
Nitric Oxide Synthase Type III - antagonists & inhibitors
Nitric Oxide Synthase Type III - metabolism
Original
Portal
Portal Pressure - drug effects
Protein Kinase Inhibitors - pharmacology
Rats, Sprague-Dawley
Receptor, Endothelin B - drug effects
Receptor, Endothelin B - metabolism
Signal Transduction - drug effects
Splanchnic Circulation - drug effects
Time Factors
Vasoconstriction - drug effects
Vasoconstrictor Agents - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:AIM: To investigate the effects of Glytan on splanchnic hemodynamics and its reduction of portal pressure in portal hypertensive rats.METHODS:Glytan(Ganluotong in Chinese),is composed of salvianolic acid B and diammonium glycyrrhizinate.Portal hypertension(PHT)was induced in the rats by common bile duct ligation(BDL).Hemodynamic studies were performed using the colored microsphere method.Radioimmunoassay(RIA)was used to determine endothelin(ET)-1 levels in the mesenteric circulation.Western blotting methods were used to investigate the effect of Glytan on ET A receptor(ETAR),ET B receptor(ETBR),endothelial NO synthase(e NOS),G-protein-coupled receptor kinase(GRK)2,and β-arrestin 2 expression in the mesentery.The m RNA of ETAR and ETBR was determined using real-time polymerase chain reaction.RESULTS:Treatment with Glytan reduced portal pressure(PP)and portal territory blood flow(PTBF)and increased both mean arterial pressure(MAP)and splanchnic vascular resistance(SVR).Especially at 4wk,PP decreased by about 40%,while MAP increased by 13%,SVR increased by 12%,and PTBF decreased by about 21%.The effect of blood flow reduction was greatest in the mesentery(about 33%)at 4 wk.The mesenteric circulation ET-1 levels of BDL rats were lower and negatively correlated with PP at 4 wk.Glytan can increase mesenteric ET-1 content and inhibit ETBR,e NOS,GRK2,andβ-arrestin 2 expression in the mesentery.Moreover,Glytan showed no effect on the expression of ETAR protein and m RNA.CONCLUSION:The decreased PP and PTBF observed after Glytan treatment were related to increased mesenteric vasoconstriction and increased receptor sensitivity to vasoconstrictor.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v20.i44.16674