Prevalence of methicillin-resistant Staphylococcus haemolyticus in companion animals: a cross-sectional study

Among coagulase-negative staphylococci, Staphylococcus haemolyticus is the second most frequently isolated species from human blood cultures and has the highest level of antimicrobial resistance. This species has zoonotic character and is prevalent both in humans and animals. Recent studies have ind...

Full description

Saved in:
Bibliographic Details
Published in:Annals of clinical microbiology and antimicrobials 2014-11, Vol.13 (1), p.56-56, Article 56
Main Authors: Ruzauskas, Modestas, Siugzdiniene, Rita, Klimiene, Irena, Virgailis, Marius, Mockeliunas, Raimundas, Vaskeviciute, Lina, Zienius, Dainius
Format: Article
Language:English
Subjects:
Aminoglycosides
Analysis
Animals
Anti-Bacterial Agents - pharmacology
Antibiotics
Antimicrobial agents
Bacteria
Blood
Carrier State - epidemiology
Carrier State - microbiology
Cats
Cloning
Cluster Analysis
Cross-Sectional Studies
Desorption
DNA Fingerprinting
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Dogs
Drug resistance
Drug resistance in microorganisms
Electrophoresis, Gel, Pulsed-Field
Enzymes
Females
Genes, Bacterial
Genotype
Health aspects
Health sciences
Horses
Humans
Identification systems
Intensive care
Laboratories
Mass spectrometry
Medical examination
Methicillin Resistance
Microbial Sensitivity Tests
Pets - microbiology
Polymerase Chain Reaction
Prevalence
Risk factors
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Staphylococcal Infections - epidemiology
Staphylococcal Infections - microbiology
Staphylococcus
Staphylococcus haemolyticus
Staphylococcus haemolyticus - classification
Staphylococcus haemolyticus - drug effects
Staphylococcus haemolyticus - genetics
Staphylococcus haemolyticus - isolation & purification
Staphylococcus infections
Studies
Veterinary medicine
Zoonoses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Among coagulase-negative staphylococci, Staphylococcus haemolyticus is the second most frequently isolated species from human blood cultures and has the highest level of antimicrobial resistance. This species has zoonotic character and is prevalent both in humans and animals. Recent studies have indicated that methicillin-resistant S. haemolyticus (MRSH) is one of the most frequent isolated Staphylococcus species among neonates in intensive care units. The aim of this study was to determine the presence of MRSH in different groups of companion animals and to characterize isolates according their antimicrobial resistance. Samples (n = 754) were collected from healthy and diseased dogs and cats, female dogs in pure-breed kennels, healthy horses, and kennel owners. Classical microbiological tests along with molecular testing including PCR and 16S rRNA sequencing were performed to identify MRSH. Clonality of the isolates was assessed by Pulsed Field Gel Electrophoresis using the SmaI restriction enzyme. Antimicrobial susceptibility testing was performed using the broth micro-dilution method. Detection of genes encoding antimicrobial resistance was performed by PCR. Statistical analysis was performed using the R Project of Statistical Computing, "R 1.8.1" package. From a total of 754 samples tested, 12 MRSH isolates were obtained. No MRSH were found in horses and cats. Eleven isolates were obtained from dogs and one from a kennel owner. Ten of the dog isolates were detected in pure-breed kennels. The isolates demonstrated the same clonality only within separate kennels.The most frequent resistances of MRSH isolates was demonstrated to benzylpenicillin (91.7%), erythromycin (91.7%), gentamicin (75.0%), tetracycline (66.7%), fluoroquinolones (41.7%) and co-trimoxazole (41.7%). One isolate was resistant to streptogramins. All isolates were susceptible to daptomycin, rifampin, linezolid and vancomycin. The clone isolated from the kennel owner and one of the dogs was resistant to beta-lactams, macrolides, gentamicin and tetracycline. Pure-breed kennels keeping 6 or more females were determined to be a risk factor for the presence of MRSH strains. MRSH isolated from companion animals were frequently resistant to some classes of critically important antimicrobials, although they remain susceptible to antibiotics used exclusively in human medicine.
ISSN:1476-0711
1476-0711
DOI:10.1186/s12941-014-0056-y