Molecular mechanism of formation of cortical opacity in CRYAAN101D transgenic mice

The CRYAAN101D transgenic mouse model expressing deamidated αA-crystallin (deamidation at N101 position to D) develops cortical cataract at the age of 7 to 9 months. The present study was carried out to explore the molecular mechanism that leads to the development of cortical opacity in CRYAAN101D l...

Full description

Saved in:
Bibliographic Details
Published in:Investigative ophthalmology & visual science 2014-10, Vol.55 (10), p.6398-6408
Main Authors: Hegde, Shylaja M, Srivastava, Kiran, Tiwary, Ekta, Srivastava, Om P
Format: Article
Language:English
Subjects:
alpha-Crystallin A Chain - biosynthesis
alpha-Crystallin A Chain - genetics
Animals
Blotting, Western
Cataract - genetics
Cataract - metabolism
Cataract - pathology
Cell Proliferation
Disease Models, Animal
Gene Expression Regulation
Immunohistochemistry
Lens Cortex, Crystalline - metabolism
Lens Cortex, Crystalline - pathology
Mice
Mice, Transgenic
RNA, Messenger - genetics
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The CRYAAN101D transgenic mouse model expressing deamidated αA-crystallin (deamidation at N101 position to D) develops cortical cataract at the age of 7 to 9 months. The present study was carried out to explore the molecular mechanism that leads to the development of cortical opacity in CRYAAN101D lenses. RNA sequence analysis was carried out on 2- and 4-month-old αA-N101D and wild type (WT) lenses. To understand the biologic relevance and function of significantly altered genes, Ingenuity Pathway Analysis (IPA) was done. To elucidate terminal differentiation defects, immunohistochemical, and Western blot analyses were carried out. RNA sequence and IPA data suggested that the genes belonging to gene expression, cellular assembly and organization, and cell cycle and apoptosis networks were altered in N101D lenses. In addition, the tight junction signaling and Rho A signaling were among the top three canonical pathways that were affected in N101D mutant. Immunohistochemical analysis identified a series of terminal differentiation defects in N101D lenses, specifically, increased proliferation and decreased differentiation of lens epithelial cells (LEC) and decreased denucleation of lens fiber cells (LFC). The expression of Rho A was reduced in different-aged N101D lenses, and, conversely, Cdc42 and Rac1 expressions were increased in the N101D mutants. Moreover, earlier in development, the expression of major membrane-bound molecular transporter Na,K-ATPase was drastically reduced in N101D lenses. The results suggest that the terminal differentiation defects, specifically, increased proliferation and decreased denucleation are responsible for the development of lens opacity in N101D lenses.
ISSN:1552-5783
0146-0404
1552-5783
DOI:10.1167/iovs.14-14623