Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species

The genomes of three strains of Listeria monocytogenes that have been associated with food-borne illness in the USA were subjected to whole genome comparative analysis. A total of 51, 97 and 69 strain-specific genes were identified in L.monocytogenes strains F2365 (serotype 4b, cheese isolate), F685...

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Bibliographic Details
Published in:Nucleic acids research 2004, Vol.32 (8), p.2386-2395
Main Authors: Nelson, K.E, Fouts, D.E, Mongodin, E.F, Ravel, J, DeBoy, R.T, Kolonay, J.F, Rasko, D.A, Angiuoli, S.V, Gill, S.R, Paulsen, I.T, Wonderling, L.D, Uhlich, G.A, Bayles, D.O, Luchansky, J.B
Format: Article
Language:English
Subjects:
Base Composition
cheeses
Chromosomes, Bacterial - genetics
DNA Transposable Elements - genetics
food contamination
Food Microbiology
food pathogens
genes
Genes, Bacterial - genetics
genome
Genome, Bacterial
Genomics
hot dogs
Listeria monocytogenes
Listeria monocytogenes - classification
Listeria monocytogenes - genetics
Listeria monocytogenes - metabolism
Meat - microbiology
microbial contamination
molecular sequence data
mutation
nucleotide sequences
Open Reading Frames - genetics
phage insertions
Physical Chromosome Mapping
Polymorphism, Single Nucleotide - genetics
prophages
Prophages - genetics
serotypes
Serotyping
single nucleotide polymorphism
Species Specificity
strain differences
strains
Synteny
transposons
turkey meat
Virulence - genetics
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Summary:The genomes of three strains of Listeria monocytogenes that have been associated with food-borne illness in the USA were subjected to whole genome comparative analysis. A total of 51, 97 and 69 strain-specific genes were identified in L.monocytogenes strains F2365 (serotype 4b, cheese isolate), F6854 (serotype 1/2a, frankfurter isolate) and H7858 (serotype 4b, meat isolate), respectively. Eighty-three genes were restricted to serotype 1/2a and 51 to serotype 4b strains. These strain- and serotype-specific genes probably contribute to observed differences in pathogenicity, and the ability of the organisms to survive and grow in their respective environmental niches. The serotype 1/2a-specific genes include an operon that encodes the rhamnose biosynthetic pathway that is associated with teichoic acid biosynthesis, as well as operons for five glycosyl transferases and an adenine-specific DNA methyltransferase. A total of 8603 and 105 050 high quality single nucleotide polymorphisms (SNPs) were found on the draft genome sequences of strain H7858 and strain F6854, respectively, when compared with strain F2365. Whole genome comparative analyses revealed that the L.monocytogenes genomes are essentially syntenic, with the majority of genomic differences consisting of phage insertions, transposable elements and SNPs.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/gkh562