Extraction and quantification of phenolic acids and flavonols from Eugenia pyriformis using different solvents

The recovery of phenolic compounds of Eugenia pyriformis using different solvents was investigated in this study. The compounds were identified and quantified by reverse-phase high-performance liquid chromatography coupled with ultraviolet–visible diode-array detector (RP-HPLC-DAD/UV–vis). Absolute...

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Bibliographic Details
Published in:Journal of food science and technology 2014-10, Vol.51 (10), p.2862-2866
Main Authors: Haminiuk, Charles Windson Isidoro, Plata-Oviedo, Manuel Salvador Vicente, de Mattos, Gisely, Carpes, Solange Teresinha, Branco, Ivanise Guilherme
Format: Article
Language:English
Subjects:
Acids
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Chromatography
Ethanol
Food
Food Science
Fruits
Functional foods & nutraceuticals
Nutrition
Original
Original Article
Phenols
Polyphenols
Solvent extraction processes
Solvents
Statistical analysis
Studies
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Summary:The recovery of phenolic compounds of Eugenia pyriformis using different solvents was investigated in this study. The compounds were identified and quantified by reverse-phase high-performance liquid chromatography coupled with ultraviolet–visible diode-array detector (RP-HPLC-DAD/UV–vis). Absolute methanol was the most effective extraction agent of phenolic acids and flavonols (588.31 mg/Kg) from Eugenia pyriformis , although similar results ( p  ≤ 0.05) were observed using methanol/water (1:1 ratio). Our results clearly showed that higher contents of phenolic compounds were not obtained either with the most or the least polar solvents used. Several phenolic compounds were identified in the samples whereas gallic acid and quercetin were the major compounds recovered.
ISSN:0022-1155
0975-8402
DOI:10.1007/s13197-012-0759-z