Persistent Luminescence Strontium Aluminate Nanoparticles as Reporters in Lateral Flow Assays

Demand for highly sensitive, robust diagnostics and environmental monitoring methods has led to extensive research in improving reporter technologies. Inorganic phosphorescent materials exhibiting persistent luminescence are commonly found in electroluminescent displays and glowing paints but are no...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2014-10, Vol.86 (19), p.9481-9488
Main Authors: Paterson, Andrew S, Raja, Balakrishnan, Garvey, Gavin, Kolhatkar, Arati, Hagström, Anna E. V, Kourentzi, Katerina, Lee, T. Randall, Willson, Richard C
Format: Article
Language:English
Subjects:
Aluminates
Aluminum - chemistry
Animals
Antibodies, Monoclonal - chemistry
Assaying
Avidin - chemistry
Bioassays
Biochemistry
Biological Assay
Biotin - chemistry
Biotinylation
Carbodiimides - chemistry
Chickens
Diagnostic systems
Environmental monitoring
Gold Colloid - chemistry
Limit of Detection
Luminescence
Metal Nanoparticles - chemistry
Muramidase - analysis
Muramidase - chemistry
Nanoparticles
Photoluminescence
Proteins
Rare earth metals
Silicon Dioxide - chemistry
Strontium
Strontium - chemistry
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Summary:Demand for highly sensitive, robust diagnostics and environmental monitoring methods has led to extensive research in improving reporter technologies. Inorganic phosphorescent materials exhibiting persistent luminescence are commonly found in electroluminescent displays and glowing paints but are not widely used as reporters in diagnostic assays. Persistent luminescence nanoparticles (PLNPs) offer advantages over conventional photoluminescent probes, including the potential for enhanced sensitivity by collecting time-resolved measurements or images with decreased background autofluorescence while eliminating the need for expensive optical hardware, superior resistance to photobleaching, amenability to quantitation, and facile bioconjugation schemes. We isolated rare-earth doped strontium aluminate PLNPs from larger-particle commercial materials by wet milling and differential sedimentation and water-stabilized the particles by silica encapsulation using a modified Stöber process. Surface treatment with aldehyde silane followed by reductive amination with heterobifunctional amine-poly­(ethylene glycol)-carboxyl allowed covalent attachment of proteins to the particles using standard carbodiimide chemistry. NeutrAvidin PLNPs were used in lateral flow assays (LFAs) with biotinylated lysozyme as a model analyte in buffer and monoclonal anti-lysozyme HyHEL-5 antibodies at the test line. Preliminary experiments revealed a limit of detection below 100 pg/mL using the NeutrAvidin PLNPs, which was approximately an order of magnitude more sensitive than colloidal gold.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac5012624