Biomimetic Chemical Sensors Using Nanoelectronic Readout of Olfactory Receptor Proteins

We have designed and implemented a practical nanoelectronic interface to G-protein coupled receptors (GPCRs), a large family of membrane proteins whose roles in the detection of molecules outside eukaryotic cells make them important pharmaceutical targets. Specifically, we have coupled olfactory rec...

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Published in:ACS nano 2011-07, Vol.5 (7), p.5408-5416
Main Authors: Goldsmith, Brett R, Mitala, Joseph J, Josue, Jesusa, Castro, Ana, Lerner, Mitchell B, Bayburt, Timothy H, Khamis, Samuel M, Jones, Ryan A, Brand, Joseph G, Sligar, Stephen G, Luetje, Charles W, Gelperin, Alan, Rhodes, Paul A, Discher, Bohdana M, Johnson, A. T. Charlie
Format: Article
Language:English
Subjects:
Animals
Binding
Biomimetics - instrumentation
Biosensing Techniques - instrumentation
Coupling (molecular)
Devices
Electrical Equipment and Supplies
Electronics
HEK293 Cells
Humans
Mice
Nanostructure
Nanotechnology - instrumentation
Nanotubes, Carbon - chemistry
Nose
Proteins
Receptors
Receptors, Odorant - metabolism
Transistors, Electronic
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cited_by cdi_FETCH-LOGICAL-a503t-e2224ba91356def070c9956a6236235ddd823d798e1cfc63792c4265af5a39953
cites cdi_FETCH-LOGICAL-a503t-e2224ba91356def070c9956a6236235ddd823d798e1cfc63792c4265af5a39953
container_end_page 5416
container_issue 7
container_start_page 5408
container_title ACS nano
container_volume 5
creator Goldsmith, Brett R
Mitala, Joseph J
Josue, Jesusa
Castro, Ana
Lerner, Mitchell B
Bayburt, Timothy H
Khamis, Samuel M
Jones, Ryan A
Brand, Joseph G
Sligar, Stephen G
Luetje, Charles W
Gelperin, Alan
Rhodes, Paul A
Discher, Bohdana M
Johnson, A. T. Charlie
description We have designed and implemented a practical nanoelectronic interface to G-protein coupled receptors (GPCRs), a large family of membrane proteins whose roles in the detection of molecules outside eukaryotic cells make them important pharmaceutical targets. Specifically, we have coupled olfactory receptor proteins (ORs) with carbon nanotube transistors. The resulting devices transduce signals associated with odorant binding to ORs in the gas phase under ambient conditions and show responses that are in excellent agreement with results from established assays for OR–ligand binding. The work represents significant progress on a path toward a bioelectronic nose that can be directly compared to biological olfactory systems as well as a general method for the study of GPCR function in multiple domains using electronic readout.
doi_str_mv 10.1021/nn200489j
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Animals
Binding
Biomimetics - instrumentation
Biosensing Techniques - instrumentation
Coupling (molecular)
Devices
Electrical Equipment and Supplies
Electronics
HEK293 Cells
Humans
Mice
Nanostructure
Nanotechnology - instrumentation
Nanotubes, Carbon - chemistry
Nose
Proteins
Receptors
Receptors, Odorant - metabolism
Transistors, Electronic
title Biomimetic Chemical Sensors Using Nanoelectronic Readout of Olfactory Receptor Proteins
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