Biomimetic Chemical Sensors Using Nanoelectronic Readout of Olfactory Receptor Proteins

We have designed and implemented a practical nanoelectronic interface to G-protein coupled receptors (GPCRs), a large family of membrane proteins whose roles in the detection of molecules outside eukaryotic cells make them important pharmaceutical targets. Specifically, we have coupled olfactory rec...

Full description

Saved in:
Bibliographic Details
Published in:ACS nano 2011-07, Vol.5 (7), p.5408-5416
Main Authors: Goldsmith, Brett R, Mitala, Joseph J, Josue, Jesusa, Castro, Ana, Lerner, Mitchell B, Bayburt, Timothy H, Khamis, Samuel M, Jones, Ryan A, Brand, Joseph G, Sligar, Stephen G, Luetje, Charles W, Gelperin, Alan, Rhodes, Paul A, Discher, Bohdana M, Johnson, A. T. Charlie
Format: Article
Language:English
Subjects:
Animals
Binding
Biomimetics - instrumentation
Biosensing Techniques - instrumentation
Coupling (molecular)
Devices
Electrical Equipment and Supplies
Electronics
HEK293 Cells
Humans
Mice
Nanostructure
Nanotechnology - instrumentation
Nanotubes, Carbon - chemistry
Nose
Proteins
Receptors
Receptors, Odorant - metabolism
Transistors, Electronic
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have designed and implemented a practical nanoelectronic interface to G-protein coupled receptors (GPCRs), a large family of membrane proteins whose roles in the detection of molecules outside eukaryotic cells make them important pharmaceutical targets. Specifically, we have coupled olfactory receptor proteins (ORs) with carbon nanotube transistors. The resulting devices transduce signals associated with odorant binding to ORs in the gas phase under ambient conditions and show responses that are in excellent agreement with results from established assays for OR–ligand binding. The work represents significant progress on a path toward a bioelectronic nose that can be directly compared to biological olfactory systems as well as a general method for the study of GPCR function in multiple domains using electronic readout.
ISSN:1936-0851
1936-086X
DOI:10.1021/nn200489j