NeuCode Labels for Relative Protein Quantification

We describe a synthesis strategy for the preparation of lysine isotopologues that differ in mass by as little as 6 mDa. We demonstrate that incorporation of these molecules into the proteomes of actively growing cells does not affect cellular proliferation, and we discuss how to use the embedded mas...

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Bibliographic Details
Published in:Molecular & cellular proteomics 2014-09, Vol.13 (9), p.2503-2512
Main Authors: Merrill, Anna E., Hebert, Alexander S., MacGilvray, Matthew E., Rose, Christopher M., Bailey, Derek J., Bradley, Joel C., Wood, William W., El Masri, Marwan, Westphall, Michael S., Gasch, Audrey P., Coon, Joshua J.
Format: Article
Language:English
Subjects:
Lysine - metabolism
Proteome
Proteomics - methods
Saccharomyces cerevisiae Proteins - analysis
Saccharomyces cerevisiae Proteins - drug effects
Saccharomyces cerevisiae Proteins - metabolism
Sodium Chloride - pharmacology
Stress, Physiological - physiology
Technological Innovation and Resources
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Summary:We describe a synthesis strategy for the preparation of lysine isotopologues that differ in mass by as little as 6 mDa. We demonstrate that incorporation of these molecules into the proteomes of actively growing cells does not affect cellular proliferation, and we discuss how to use the embedded mass signatures (neutron encoding (NeuCode)) for multiplexed proteome quantification by means of high-resolution mass spectrometry. NeuCode SILAC amalgamates the quantitative accuracy of SILAC with the multiplexing of isobaric tags and, in doing so, offers up new opportunities for biological investigation. We applied NeuCode SILAC to examine the relationship between transcript and protein levels in yeast cells responding to environmental stress. Finally, we monitored the time-resolved responses of five signaling mutants in a single 18-plex experiment.
ISSN:1535-9476
1535-9484
DOI:10.1074/mcp.M114.040287