Molecular detection of monocyte chemotactic protein-1 polymorphism in spontaneous bacterial peritonitis patients

AIM: To investigate the association of the functional monocyte chemotactic protein-1(MCP-1) promoter polymorphism(A-2518G) with spontaneous bacterial peritonitis(SBP).METHODS: Fifty patients with post-hepatitis C liver cirrhosis and ascites were categorized into two groups; group Ⅰ included 25 patie...

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Published in:World journal of gastroenterology : WJG 2014-09, Vol.20 (33), p.11793-11799
Main Authors: Salama, Maysa Kamal, Sabry, Dina, Al-Ghussein, Mohamed A S, Ahmed, Rasha, AbdAllah, Sayed, Taha, Fatma Mohamed, Fathy, Wael, Wadie, Miriam Safwat, Nabih, Mona, Abul-Fotouh, Amr, Darwish, Tarneem
Format: Article
Language:English
Subjects:
Adult
Ascites - immunology
Bacterial Infections - diagnosis
Bacterial Infections - genetics
Bacterial Infections - immunology
Bacterial Infections - microbiology
Bacterial Infections - therapy
Biomarkers - blood
Case Control Study
Case-Control Studies
Chemokine CCL2 - genetics
chemotactic
Female
Gene Frequency
Genetic Predisposition to Disease
Genotype
Hepatitis C - complications
Humans
Interleukin-10 - blood
Male
Middle Aged
Monocyte
Peritonitis - diagnosis
Peritonitis - genetics
Peritonitis - immunology
Peritonitis - microbiology
Peritonitis - therapy
Phenotype
Polymorphism, Genetic
Promoter Regions, Genetic
protein-1
Risk Factors
Spontaneou
Treatment Outcome
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Summary:AIM: To investigate the association of the functional monocyte chemotactic protein-1(MCP-1) promoter polymorphism(A-2518G) with spontaneous bacterial peritonitis(SBP).METHODS: Fifty patients with post-hepatitis C liver cirrhosis and ascites were categorized into two groups; group Ⅰ included 25 patients with SBP and group Ⅱ included 25 patients free from SBP. In addition, a group of 20 healthy volunteers were included. We assessed the MCP-1 gene polymorphism and gene expression as well as interleukin(IL)-10 levels in both blood and ascitic fluid. RESULTS: A significant MCP-1 gene polymorphism was detected in groups Ⅰ and Ⅱ(P = 0.001 and 0.02 respectively). Group Ⅰ was associated with a significantly higher frequency of AG genotype [control 8(40%) vs SBP 19(76.0%), P < 0.001], and group Ⅱ was associated with a significantly higher frequency of GG genotype when compared to healthy volunteers [control 1(5%) vs cirrhotic 16(64%), P < 0.001]. Accordingly, the frequency of G allele was significantly higher in both groups(Ⅰ and Ⅱ) [control 10(25%) vs SBP 27(54%), P < 0.001 and vs cirrhotic 37(74.0%), P < 0.001, respectively]. The total blood and ascetic fluid levels of IL-10 and MCP-1 gene expression were significantly higher in group Ⅰ than in group Ⅱ. Group Ⅰ showed significant reductions in the levels of MCP-1 gene expression and IL-10 in the whole blood and ascetic fluid after therapy. CONCLUSION: MCP-1 GG genotype and G allele may predispose HCV infected patients to a more progressive disease course, while AG genotype may increase the susceptibility to SBP. Patients carrying these genotypes should be under supervision to prevent or restrict further complications.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v20.i33.11793