Decreased Gene Expressions of Insulin Signal Molecules in Canine Hyperadrenocorticism

Hyperadrenocorticism (HAC) is a common endocrine disorder in dogs, in which excess glucocorticoid causes insulin resistance. Disturbance of insulin action may be caused by multiple factors, including transcriptional modulation of insulin signal molecules which lie downstream of insulin binding to in...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2014, Vol.76(8), pp.1177-1182
Main Authors: NOZAWA, Satoshi, ODA, Hitomi, AKIYAMA, Ran, UEDA, Kaori, SAEKI, Kaori, SHONO, Saori, MARUYAMA, Natsuki, MURATA, Atsuki, TAZAKI, Hiroyuki, MORI, Akihiro, MOMOTA, Yutaka, AZAKAMI, Daigo, SAKO, Toshinori, ISHIOKA, Katsumi
Format: Article
Language:English
Subjects:
Adrenocortical Hyperfunction - drug therapy
Adrenocortical Hyperfunction - metabolism
Adrenocortical Hyperfunction - veterinary
Animals
canine
Dihydrotestosterone - analogs & derivatives
Dihydrotestosterone - therapeutic use
DNA Primers - genetics
Dog Diseases - drug therapy
Dog Diseases - metabolism
Dogs
Female
Gene Expression Regulation - physiology
hyperadrenocorticism
Insulin Receptor Substrate Proteins - metabolism
Insulin Resistance - physiology
insulin signal molecules
Internal Medicine
Isoenzymes - metabolism
Male
neutrophils
Neutrophils - metabolism
Phosphatidylinositol 3-Kinase - metabolism
Protein Kinase C - metabolism
Proto-Oncogene Proteins c-akt - metabolism
Statistics, Nonparametric
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Summary:Hyperadrenocorticism (HAC) is a common endocrine disorder in dogs, in which excess glucocorticoid causes insulin resistance. Disturbance of insulin action may be caused by multiple factors, including transcriptional modulation of insulin signal molecules which lie downstream of insulin binding to insulin receptors. In this study, gene expressions of insulin signal molecules were examined using neutrophils of the HAC dogs (the untreated dogs and the dogs which had been treated with trilostane). Insulin receptor substrate (IRS)-1, IRS-2, phosphatidylinositol 3-kinase (PI3-K), protein kinase B/Akt kinase (Akt)-2 and protein kinase C (PKC)-lambda were analyzed in the HAC dogs and compared with those from normal dogs. The IRS-1 gene expressions decreased by 37% and 35% of the control dogs in the untreated and treated groups, respectively. The IRS-2 gene expressions decreased by 61% and 72%, the PI3-K gene expressions decreased by 47% and 55%, and the Akt-2 gene expressions decreased by 45% and 56% of the control dogs, similarly. Collectively, gene expressions of insulin signal molecules are suppressed in the HAC dogs, which may partially contribute to the induction of insulin resistance.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.14-0033