Effective Stimulation of the Biotechnological Potential of the Medicinal White Rot Fungus: Phellinus pini by Menadione-Mediated Oxidative Stress

The effect of menadione (MQ; 2-methyl-1,4-naphtoquinone), a superoxide-generating agent, on the natural biodegradation system in the medicinal white rot fungus Phellinus pini was determined. While measuring the activities of extracellular manganese-dependent peroxidase (MnP) and intracellular chitin...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2014-09, Vol.174 (2), p.644-656
Main Authors: Jaszek, Magdalena, Kos, Katarzyna, Matuszewska, Anna, Gr z, Marcin, Stefaniuk, Dawid, Osińska-Jaroszuk, Monika, Prendecka, Monika, Jóźwik, Ewa, Grzywnowicz, Krzysztof
Format: Article
Language:English
Subjects:
Basidiomycota - metabolism
Biochemistry
biodegradation
Biological and medical sciences
Biotechnology
capillary electrophoresis
catalase
Chemistry
Chemistry and Materials Science
chitinase
Electrophoresis, Capillary
formaldehyde
Fundamental and applied biological sciences. Psychology
Fungi
Hydrogen-Ion Concentration
menadione
oxalic acid
Oxidative stress
Oxidative Stress - drug effects
peroxidase
Phellinus pini
phenolic compounds
Proteolysis
serine proteinases
superoxide anion
Vitamin K 3 - pharmacology
white-rot fungi
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Summary:The effect of menadione (MQ; 2-methyl-1,4-naphtoquinone), a superoxide-generating agent, on the natural biodegradation system in the medicinal white rot fungus Phellinus pini was determined. While measuring the activities of extracellular manganese-dependent peroxidase (MnP) and intracellular chitinase, it was found that the application of MQ (0.75 mM) distinctly stimulated the activities of these enzymes in comparison to the control values (without MQ). Using the capillary electrophoresis (CE) method, an increase in the extracellular oxalic acid (OXA) concentration was detected during the first days after the addition of MQ. It was observed that the rate of intracellular proteolysis at pH 3.5 evidently decreased under oxidative stress conditions. Contrary to these results, the activities of serine proteases at pH 9.5 measured against fluorogenic peptide substrates distinctly increased in stressed cultures. The MQ treatment also caused an evident increase in the catalase (CAT) activity, as well as the levels of superoxide anion radicals (SORs), formaldehyde (FA), and phenolic compounds (PHC) in the experimental cultures. The results obtained confirm that prooxidants may find application as an effective way to stimulate biotechnological production of MnP and chitinase by white rot fungi.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-014-1064-2