Ursolic acid induces neural regeneration after sciatic nerve injury

In this study, we aimed to explore the role of ursolic acid in the neural regeneration of the injured sciatic nerve. BALB/c mice were used to establish models of sciatic nerve injury through unilateral sciatic nerve complete transection and microscopic anastomosis at 0.5 cm below the ischial tuber-o...

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Bibliographic Details
Published in:Neural regeneration research 2013-09, Vol.8 (27), p.2510-2519
Main Authors: Liu, Biao, Liu, Yan, Yang, Guang, Xu, Zemin, Chen, Jiajun
Format: Article
Language:English
Subjects:
Acids
Animal experimentation
Antigens
Care and treatment
Cytokines
Gene expression
Immunoglobulins
Injuries
Lymphocytes
Nervous system
Physiological aspects
Regeneration
Research and Report : Sciatic Nerve Injury and Neural Regeneration
S100蛋白
Sciatic nerve
Spinal cord
乌索酸
大鼠
实时定量PCR
神经再生
神经损伤
诱导
酶联免疫吸附试验
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Summary:In this study, we aimed to explore the role of ursolic acid in the neural regeneration of the injured sciatic nerve. BALB/c mice were used to establish models of sciatic nerve injury through unilateral sciatic nerve complete transection and microscopic anastomosis at 0.5 cm below the ischial tuber-osity. The successful y generated model mice were treated with 10, 5, or 2.5 mg/kg ursolic acid via intraperitoneal injection. Enzyme-linked immunosorbent assay results showed that serum S100 protein expression level gradual y increased at 1-4 weeks after sciatic nerve injury, and significantly decreased at 8 weeks. As such, ursolic acid has the capacity to significantly increase S100 protein expression levels. Real-time quantitative PCR showed that S100 mRNA expression in the L 4-6 segments on the injury side was increased after ursolic acid treatment. In addition, the muscular mass index in the soleus muscle was also increased in mice treated with ursolic acid. Toluidine blue staining revealed that the quantity and average diameter of myelinated nerve fibers in the injured sciatic nerve were significantly increased after treatment with ursolic acid. 10 and 5 mg/kg of ursolic acid produced stronger effects than 2.5 mg/kg of ursolic acid. Our findings indicate that ursolic acid can dose-dependently increase S100 expression and promote neural regeneration in BALB/c mice fol owing sciatic nerve injury.
ISSN:1673-5374
1876-7958
DOI:10.3969/j.issn.1673-5374.2013.27.002