Reconstituting ring-rafts in bud-mimicking topography of model membranes

During vesicular trafficking and release of enveloped viruses, the budding and fission processes dynamically remodel the donor cell membrane in a protein- or a lipid-mediated manner. In all cases, in addition to the generation or relief of the curvature stress, the buds recruit specific lipids and p...

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Bibliographic Details
Published in:Nature communications 2014-07, Vol.5 (1), p.4507-4507, Article 4507
Main Authors: Ryu, Yong-Sang, Lee, In-Ho, Suh, Jeng-Hun, Park, Seung Chul, Oh, Soojung, Jordan, Luke R, Wittenberg, Nathan J, Oh, Sang-Hyun, Jeon, Noo Li, Lee, Byoungho, Parikh, Atul N, Lee, Sin-Doo
Format: Article
Language:English
Subjects:
Cell Membrane - chemistry
Cell Membrane - metabolism
Cholesterol - chemistry
Cholesterol - metabolism
Dimethylpolysiloxanes - chemistry
Imaging, Three-Dimensional
Lipid Bilayers - chemistry
MATERIALS SCIENCE
Membrane Lipids - chemistry
Membrane Lipids - metabolism
Membrane Microdomains - chemistry
Membrane Microdomains - metabolism
Phosphatidylcholines - chemistry
Science & Technology - Other Topics
Sphingomyelins - chemistry
Sphingomyelins - metabolism
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Summary:During vesicular trafficking and release of enveloped viruses, the budding and fission processes dynamically remodel the donor cell membrane in a protein- or a lipid-mediated manner. In all cases, in addition to the generation or relief of the curvature stress, the buds recruit specific lipids and proteins from the donor membrane through restricted diffusion for the development of a ring-type raft domain of closed topology. Here, by reconstituting the bud topography in a model membrane, we demonstrate the preferential localization of cholesterol- and sphingomyelin-enriched microdomains in the collar band of the bud-neck interfaced with the donor membrane. The geometrical approach to the recapitulation of the dynamic membrane reorganization, resulting from the local radii of curvatures from nanometre-to-micrometre scales, offers important clues for understanding the active roles of the bud topography in the sorting and migration machinery of key signalling proteins involved in membrane budding.
ISSN:2041-1723
2041-1723
DOI:10.1038/ncomms5507